Super-Resolution Microscopy

1. Front Matter

   Pages i-xi

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2. Super-Resolution Microscopy Techniques and Their Potential for Applications in Radiation Biophysics

   • Jan Philipp Eberle, Alexander Rapp, Matthias Krufczik, Marion Eryilmaz, Manuel Gunkel, Holger Erfle et al.

   Pages 1-13

3. Managing the Introduction of Super-Resolution Microscopy into a Core Facility

   • Jeffrey A. Kamykowski, Brian Storrie

   Pages 15-19

4. Live-Cell STED Imaging with the HyPer2 Biosensor

   • Natalia M. Mishina, Vsevolod V. Belousov

   Pages 21-28

5. Diffraction-Unlimited Fluorescence Imaging with an EasySTED Retrofitted Confocal Microscope

   • André Klauss, Carsten Hille

   Pages 29-44

6. Two-Photon STED Microscopy for Nanoscale Imaging of Neural Morphology In Vivo

   • Mirelle J. T. ter Veer, Thomas Pfeiffer, U. Valentin Nägerl

   Pages 45-64

7. STED Imaging of Golgi Dynamics with Cer-SiR: A Two-Component, Photostable, High-Density Lipid Probe for Live Cells

   • Roman S. Erdmann, Derek Toomre, Alanna Schepartz

   Pages 65-78

8. Four-Channel Super-Resolution Imaging by 3-D Structured Illumination

   • Ulrike Engel

   Pages 79-94

9. Correlative SIM-STORM Microscopy

   • O. Burri, T. Laroche, R. Guiet, A. Seitz

   Pages 95-103

10. Correlative Super-Resolution Fluorescence Imaging and Atomic Force Microscopy for the Characterization of Biological Samples

    • Patricia Bondia, Santiago Casado, Cristina Flors

    Pages 105-113

11. Quantitative Single-Molecule Localization Microscopy (qSMLM) of Membrane Proteins Based on Kinetic Analysis of Fluorophore Blinking Cycles

    • Franziska Fricke, Joel Beaudouin, Sebastian Malkusch, Roland Eils, Mike Heilemann

    Pages 115-126

12. Two-Color Single-Molecule Tracking in Live Cells

    • Siegfried Hänselmann, Dirk-Peter Herten

    Pages 127-138

13. Fully Automated Targeted Confocal and Single-Molecule Localization Microscopy

    • Jan Philipp Eberle, Walter Muranyi, Holger Erfle, Manuel Gunkel

    Pages 139-152

14. Brain Slice Staining and Preparation for Three-Dimensional Super-Resolution Microscopy

    • Christopher L. German, Manasa V. Gudheti, Annette E. Fleckenstein, Erik M. Jorgensen

    Pages 153-162

15. Correlative In-Resin Super-Resolution Fluorescence and Electron Microscopy of Cultured Cells

    • Errin Johnson, Rainer Kaufmann

    Pages 163-177

16. Synthesis of Janelia Fluor HaloTag and SNAP-Tag Ligands and Their Use in Cellular Imaging Experiments

    • Jonathan B. Grimm, Timothy A. Brown, Brian P. English, Timothée Lionnet, Luke D. Lavis

    Pages 179-188

17. Measuring Nanometer Distances Between Fluorescent Labels Step-by-Step

    • Susanna Maria Früh, Ingmar Schoen

    Pages 189-203

18. Correlative Single-Molecule Localization Microscopy and Confocal Microscopy

    • Christian Soeller, Yufeng Hou, Isuru D. Jayasinghe, David Baddeley, David Crossman

    Pages 205-217

19. Correlative Fluorescence Super-Resolution Localization Microscopy and Platinum Replica EM on Unroofed Cells

    • Kem A. Sochacki, Justin W. Taraska

    Pages 219-230

20. In Situ Super-Resolution Imaging of Genomic DNA with OligoSTORM and OligoDNA-PAINT

    • Brian J. Beliveau, Alistair N. Boettiger, Guy Nir, Bogdan Bintu, Peng Yin, Xiaowei Zhuang et al.

    Pages 231-252

This volume presents current advanced technologies and methods used in super-resolution microscopy. The chapters in this book cover a wide range of topics such as introducing super-resolution microscopy into a core facility; two-photon STED microscopy for nanoscale imaging of neural morphology in vivo; correlative SIM-STORM microscopy; two-color single-molecule tracking in live cells; and correlative single molecule localization microscopy and confocal microscopy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Super-Resolution Microscopy: Methods and Protocols is a valuable resource for both established and novel researchers and users in this field.

• Stimulated Emission Depletion
• Ground State Depletion
• Photo Activated Localization
• fluorescence
• STORM

“The aim is to provide a current overview of the advanced technologies and methods in super‐resolution microscopy. The book is written for novices andexperienced investigators. It will be of much help for graduate students and postdoctoral students interested in super-resolution microscopy. The author is a credible authority in the field of super-resolution microscopy. … This book covers advanced, cutting-edge techniques related to super-resolution microscopy. The intended audience will find it of immense help.” (Omer Iqbal, Doody's Book Reviews, February, 2018)

• High-Content Analysis of the Cell (HiCell) and Advanced Biological Screening Facility, BioQuant, Heidelberg University, Heidelberg, Germany

  Holger Erfle

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