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  • © 2017

MicroRNA Technologies

Editors:

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains keynotes and implementation advice from the experts

Part of the book series: Neuromethods (NM, volume 128)

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Table of contents (15 protocols)

  1. Front Matter

    Pages i-xii
  2. Protocol for miRNA In Situ Hybridization on Mouse Spinal Cord

    • Irit Reichenstein, Eran Hornstein
    Pages 1-10
  3. Quantification of Dicer Activity in Mammalian Cell Lysates Using a Non-radioactive Fluorescence Method

    • Anna Emde, Natalia Rivkin, Mark A. Behlke, Eran Hornstein
    Pages 21-27
  4. Analysis of MicroRNAs and their Potential Targets in Human Embryonic Stem Cell-Derived Neurons Treated with the Anesthetic Propofol

    • Danielle Twaroski, Yasheng Yan, Jessica M. Olson, Mingyu Liang, Zeljko J. Bosnjak, Xiaowen Bai
    Pages 29-42
  5. MicroRNA Expression Profiling by PCR Array in 2D and 3D Differentiated Neural Culture Systems and Target Validation

    • Lara Stevanato, Caroline Hicks, Lavaniya Thanabalasundaram, John D. Sinden
    Pages 43-57
  6. Functional Analysis of Cortical Neuron Migration Using miRNA Silencing

    • Pierre-Paul Prévot, Marie-Laure Volvert, Alexander Deiters, Laurent Nguyen
    Pages 73-88
  7. Continuous Delivery of Oligonucleotides into the Brain

    • Ilya A. Vinnikov, Andrii Domanskyi, Witold Konopka
    Pages 89-117
  8. Isolation and Quantitative Analysis of Axonal Small Noncoding RNAs

    • Hak Hee Kim, Monichan Phay, Soonmoon Yoo
    Pages 147-159
  9. Back Matter

    Pages 215-216

About this book

This volume examines different approaches toward understanding the function of microRNA (miRNA) and the tools used to regulate microRNA expression in nervous systems. MicroRNA Technologies also explores newly established techniques to study the role of miRNAs in the nervous system. The chapters in this book cover topics such as: high content screening for looking at the impact of overexpressed miRNAs on primary motor neurons; miRNA expression profiling by PCR array in 2D and 3D neural culture systems; analysis of cortical neuron migration using miRNA silencing; isolating and screening subcellular miRNAs in neurons; and measuring miRNA mediated translational regulation with live cell imaging. The Neuromethods series offers chapters with key advice and procedure specifics to empower the readers to successfully achieve their own scientific and experimental goals.

Cutting-edge and practical, MicroRNA Technologies is a valuable resource for students and researchers who are interested about microRNAs in nervous systems.

Editors and Affiliations

  • Institute of Human Genetics, University of Cologne, Cologne, Germany

    Min Jeong Kye

Bibliographic Information

  • Book Title: MicroRNA Technologies

  • Editors: Min Jeong Kye

  • Series Title: Neuromethods

  • DOI: https://doi.org/10.1007/978-1-4939-7175-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • Hardcover ISBN: 978-1-4939-7173-2Published: 26 July 2017

  • Softcover ISBN: 978-1-4939-8407-7Published: 02 August 2018

  • eBook ISBN: 978-1-4939-7175-6Published: 25 July 2017

  • Series ISSN: 0893-2336

  • Series E-ISSN: 1940-6045

  • Edition Number: 1

  • Number of Pages: XII, 216

  • Number of Illustrations: 10 b/w illustrations, 10 illustrations in colour

  • Topics: Neurosciences

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 159.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access