Zymography

1. Front Matter

   Pages i-xx

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2. Introduction

   1. Front Matter

      Pages 1-1

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   2. Zymography Principles

      • Jeff Wilkesman, Liliana Kurz

      Pages 3-10

3. Endopeptidase Zymography

   1. Front Matter

      Pages 11-11

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   2. Serine Protease Zymography: Low-Cost, Rapid, and Highly Sensitive RAMA Casein Zymography

      • Hidetaro Yasumitsu

      Pages 13-24

   3. Cysteine Protease Zymography: Brief Review

      • Jeff Wilkesman

      Pages 25-31

   4. Aspartic Protease Zymography Case Study: Detection of Fungal Acid Proteases by Zymography

      • Gavin Kernaghan, Michael Mayerhofer

      Pages 33-41

   5. Detection of Aspartic Proteinase Activities Using Gel Zymography

      • Handunge Kumudu Irani Perera

      Pages 43-52

   6. MMP Activity Detection in Zymograms

      • Péter Bencsik, Monika Bartekova, Anikó Görbe, Krisztina Kiss, János Pálóczi, Jana Radosinska et al.

      Pages 53-70

   7. Characterization of Novel Collagenolytic Proteases

      • Goran Mucić, Brankica Rašković, Natalija Polović

      Pages 71-78

   8. Zymography as a Research Tool in the Study of Matrix Metalloproteinase Inhibitors

      • Zongli Ren, Juanjuan Chen, Raouf A. Khalil

      Pages 79-102

   9. Detection and Characterization of Bacterial Proteinases Using Zymography

      • Madathiparambil G. Madanan, Ambili Mechoor

      Pages 103-114

   10. A Sensitive, Rapid, and Specific Technique for the Detection of Collagenase Using Zymography

       • Shivcharan Prasad, Ipsita Roy

       Pages 115-121

4. Reverse Zymography and In Situ Zymography

   1. Front Matter

      Pages 123-123

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   2. Reverse Zymography: Overview and Pitfalls

      • Kanika Sharma, Debasish Bhattacharyya

      Pages 125-132

   3. Cell In Situ Zymography: Imaging Enzyme–Substrate Interactions

      • Aastha Chhabra, Vibha Rani

      Pages 133-143

5. 2D Zymography

   1. Front Matter

      Pages 145-145

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   2. Examination of Gelatinase Isoforms in Rodent Models of Acute Neurodegenerative Diseases Using Two-Dimensional Zymography

      • Shanyan Chen, Fanjun Meng, Zhenzhou Chen, Zhe Qu, Jiankun Cui, Zezong Gu

      Pages 147-155

   3. Two-Dimensional Zymography of Proteases from Steatotic Duck Liver

      • Jeff Wilkesman, María Fernanda Padrón, Liliana Kurz, Hervé Rémignon

      Pages 157-166

6. Special Zymography Cases

   1. Front Matter

      Pages 167-167

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This detailed volume examines the latest techniques and protocols associated with zymography, the practice of detecting enzyme activity on electrophoretic gels. Sections cover subjects such as endopeptidase zymography, reverse zymography & in situ zymography, 2D zymography, as well as a variety of special cases. Written in the highly respected Methods in Molecular Biology series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Comprehensive and practical, Zymography: Methods and Protocols serves as an ideal aid for a broad array of researchers from the fields of biological and medicinal science, as well as scientists working in clinical and diagnostic medicine, medical genetics, agricultural entomology, genetic monitoring of environmental pollution, and forensic science.

• Enzyme activity
• Electrophoretic gels
• Endopeptidase zymography
• In situ zymography
• Isozymes and allozymes
• Enzymology
• Gene markers

• Centre for Environmental, Biology and Chemistry Research, Facultad de Ciencias y Tecnología, University of Carabobo, Valencia, Venezuela

  Jeff Wilkesman

• Centre for Environmental, Biology and Chemistry Research Facultad de Ciencias y Tecnología, University of Carabobo, Valencia, Venezuela

  Liliana Kurz

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