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The Renin-Angiotensin-Aldosterone System

Methods and Protocols

  • Book
  • © 2017

Overview

Editors:
  1. Sean E. Thatcher

    1. Department of Pharmacology and Nutritional Sciences, University of Kentucky, Lexington, USA

    View editor publications

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1614)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. A Brief Introduction into the Renin-Angiotensin-Aldosterone System: New and Old Techniques

    • Sean E. Thatcher
    Pages 1-19

  3. A Color Segmentation-Based Method to Quantify Atherosclerotic Lesion Compositions with Immunostaining

    • Congqing Wu, Alan Daugherty, Hong Lu
    Pages 21-30

  4. Assessment of Protein Carbonylation and Protein Tyrosine Phosphatase (PTP) Oxidation in Vascular Smooth Muscle Cells (VSMCs) Using Immunoblotting Approaches

    • Sofia Tsiropoulou, Rhian M. Touyz
    Pages 31-46

  5. Methods for Studying the Role of RAAS in the Modulation of Vascular Repair-Relevant Functions of Stem/Progenitor Cells

    • Yagna P. R. Jarajapu
    Pages 47-59

  6. Use of a Fluorescent Substrate to Measure ACE2 Activity in the Mouse Abdominal Aorta

    • Yu Wang, Lisa A. Cassis, Sean E. Thatcher
    Pages 61-67

  7. Measuring Blood Pressure Using a Noninvasive Tail Cuff Method in Mice

    • Yu Wang, Sean E. Thatcher, Lisa A. Cassis
    Pages 69-73

  8. Blood Pressure Monitoring Using Radio Telemetry Method in Mice

    • Yu Wang, Sean E. Thatcher, Lisa A. Cassis
    Pages 75-85

  9. Characterization and Functional Phenotyping of Renal Immune Cells via Flow Cytometry

    • Nathan P. Rudemiller, Steven D. Crowley
    Pages 87-98

  10. Assessment of the Renin–Angiotensin System in Cellular Organelle: New Arenas for Study in the Mitochondria

    • Bryan A. Wilson, Mark C. Chappell
    Pages 99-121

  11. Comprehensive Assessments of Energy Balance in Mice

    • Justin L. Grobe
    Pages 123-146

  12. In Vitro Assays to Determine Smooth Muscle Cell Hypertrophy, Protein Content, and Fibrosis

    • Katherine J. Elliott, Satoru Eguchi
    Pages 147-153

  13. A New Mouse Model for Introduction of Aortic Aneurysm by Implantation of Deoxycorticosterone Acetate Pellets or Aldosterone Infusion in the Presence of High Salt

    • Shu Liu, Ming C. Gong, Zhenheng Guo
    Pages 155-163

  14. Fluorescence-Based Binding Assay for Screening Ligands of Angiotensin Receptors

    • Maiia E. Bragina, Nikolaos Stergiopulos, Rodrigo A. Fraga-Silva
    Pages 165-174

  15. A Primer to Angiotensin Peptide Isolation, Stability, and Analysis by Nano-Liquid Chromatography with Mass Detection

    • Mariola Olkowicz, Stefan Chlopicki, Ryszard T. Smolenski
    Pages 175-187

  16. Analysis of Angiotensin Metabolism in the Kidney Using Mass Spectrometry

    • Nadja Grobe, Khalid M. Elased
    Pages 189-197

  17. Erratum to: Characterization and Functional Phenotyping of Renal Immune Cells via Flow Cytometry

    • Nathan P. Rudemiller, Steven D. Crowley
    Pages E1-E1

  18. Back Matter

    Pages 199-201
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Keywords

About this book

This volume discusses protocols that aid in measuring different components in the renin-angiotensin-aldosterone system (RAAS). The book also looks at the methods used to assess angiotensin peptides and discerning the influence of RAAS components on different mammalian diseases. The chapters cover topics such as the use of fluorescent substrate to measure ACE2 activity in the mouse abdominal aorta, blood pressure monitoring using the radio telemetry method, and the analysis of angiotensin metabolism in the kidney using mass spectrometry. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting edge and comprehensive, The Renin-Angiotensin-Aldosterone Systems: Methods and Protocols is a valuable resource that providesscientists and researchers with the best approaches to examine RAAS.

Editors and Affiliations

  • Department of Pharmacology and Nutritional Sciences, University of Kentucky, Lexington, USA

    Sean E. Thatcher

Bibliographic Information

  • Book Title: The Renin-Angiotensin-Aldosterone System

  • Book Subtitle: Methods and Protocols

  • Editors: Sean E. Thatcher

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7030-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • Hardcover ISBN: 978-1-4939-7028-5Published: 13 May 2017

  • Softcover ISBN: 978-1-4939-8373-5Published: 08 August 2018

  • eBook ISBN: 978-1-4939-7030-8Published: 11 May 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 201

  • Number of Illustrations: 20 b/w illustrations, 38 illustrations in colour

  • Topics: Biochemistry, general

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