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  • © 2017

Protein Crystallography

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1607)

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Table of contents (27 protocols)

  1. Front Matter

    Pages i-xiii
  2. Protein Crystallization

    • Alexander McPherson
    Pages 17-50
  3. Crystallization of Membrane Proteins: An Overview

    • Andrii Ishchenko, Enrique E. Abola, Vadim Cherezov
    Pages 117-141
  4. Locating and Visualizing Crystals for X-Ray Diffraction Experiments

    • Michael Becker, David J. Kissick, Craig M. Ogata
    Pages 143-164
  5. Applications of X-Ray Micro-Beam for Data Collection

    • Ruslan Sanishvili, Robert F. Fischetti
    Pages 219-238
  6. Serial Synchrotron X-Ray Crystallography (SSX)

    • Kay Diederichs, Meitian Wang
    Pages 239-272
  7. Processing of XFEL Data

    • Thomas A. White
    Pages 325-347
  8. Many Ways to Derivatize Macromolecules and Their Crystals for Phasing

    • Miroslawa Dauter, Zbigniew Dauter
    Pages 349-356

About this book

This volume provides methods for modern macromolecular crystallography, including all steps leading to crystal structure determination and analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Protein Crystallography aims to ensure successful results in the further study of this vital field.


Editors and Affiliations

  • Macromolecular Crystallography Laboratory, National Cancer Institute, Frederick, USA

    Alexander Wlodawer

  • Synchrotron Radiation Research Section, MCL, National Cancer Institute, Argonne National Laboratory, Argonne, USA

    Zbigniew Dauter

  • Department of Crystallography, Faculty of Chemistry, A. Mickiewicz University, Poznan, Poland

    Mariusz Jaskolski

About the editors



Bibliographic Information

  • Book Title: Protein Crystallography

  • Book Subtitle: Methods and Protocols

  • Editors: Alexander Wlodawer, Zbigniew Dauter, Mariusz Jaskolski

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7000-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • Hardcover ISBN: 978-1-4939-6998-2Published: 02 June 2017

  • Softcover ISBN: 978-1-4939-8363-6Published: 09 June 2018

  • eBook ISBN: 978-1-4939-7000-1Published: 01 June 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 672

  • Number of Illustrations: 28 b/w illustrations, 145 illustrations in colour

  • Topics: Protein Science

Buy it now

Buying options

eBook USD 149.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 199.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 279.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access