Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 1592)
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Table of contents (20 protocols)
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Front Matter
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Food Allergen Purification and Production
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Front Matter
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Food Allergen Discovery, Detection, and Quantification
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Front Matter
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Allergenic Epitope Mapping
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Front Matter
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Keywords
About this book
This volume provides a collection of methodologies for basic research, clinical diagnosis, and treatment pertaining to food allergens, including food allergen production, purification, characterization, detection, quantification, and bioinformatics approaches to modern food allergen studies. The chapters in the book are divided into 4 parts: Part I discusses food allergen purification and production, and explores methods of producing recombinant food allergens in bacterial and yeast expression systems; Part II looks at allergen discovery, detection, and quantification covering 3 types of methods—DNA-, protein-, and cell-based methods; Part III focuses on allergenic epitope mapping; and Part IV talks about future developments concentrated around new concepts of allergenicity as an outcome of protein and food matrix interactions. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists ofthe necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, Food Allergens: Methods and Protocols is a valuable resource for immunologists, biochemists, molecular biologists, and medical doctors and students working in the food allergy field. This book is also useful for people in the food industry, legislators, food standard agencies, allergologists, pediatricians, and clinicians in the allergic diseases and immunology fields.
Editors and Affiliations
Bibliographic Information
Book Title: Food Allergens
Book Subtitle: Methods and Protocols
Editors: Jing Lin, Marcos Alcocer
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-6925-8
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media LLC 2017
Hardcover ISBN: 978-1-4939-6923-4Published: 17 March 2017
Softcover ISBN: 978-1-4939-8340-7Published: 07 August 2018
eBook ISBN: 978-1-4939-6925-8Published: 17 March 2017
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: X, 299
Number of Illustrations: 24 b/w illustrations, 20 illustrations in colour
Topics: Immunology