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Population Epigenetics

Methods and Protocols

  • Book
  • © 2017

Overview

Editors:
  1. Paul Haggarty

    1. Rowett Institute of Nutrition and Health, University of Aberdeen, Bucksburn, United Kingdom

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    You can also search for this editor in PubMed   Google Scholar

  2. Kristina Harrison

    1. Rowett Institute of Nutrition and Health, University of Aberdeen , Bucksburn, United Kingdom

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols for the study of population epigenetics
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1589)

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Table of contents (12 protocols)

  1. Front Matter

    Pages i-xii
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  2. Library Construction for High-Throughput Mobile Element Identification and Genotyping

    • Hongseok Ha, Nan Wang, Jinchuan Xing
    Pages 1-15

  3. The Design and Optimization of DNA Methylation Pyrosequencing Assays Targeting Region-Specific Repeat Elements

    • Gwen Hoad, Kristina Harrison
    Pages 17-27

  4. Determining Epigenetic Targets: A Beginner’s Guide to Identifying Genome Functionality Through Database Analysis

    • Elizabeth A. Hay, Philip Cowie, Alasdair MacKenzie
    Pages 29-45

  5. Detecting Spatial Chromatin Organization by Chromosome Conformation Capture II: Genome-Wide Profiling by Hi-C

    • Matteo Vietri Rudan, Suzana Hadjur, Tom Sexton
    Pages 47-74

  6. Quantitative Analysis of Intra-chromosomal Contacts: The 3C-qPCR Method

    • Vuthy Ea, Franck Court, Thierry Forné
    Pages 75-88

  7. 5-Hydroxymethylcytosine Profiling in Human DNA

    • John P. Thomson, Colm E. Nestor, Richard R. Meehan
    Pages 89-98

  8. Adjusting for Cell Type Composition in DNA Methylation Data Using a Regression-Based Approach

    • Meaghan J. Jones, Sumaiya A. Islam, Rachel D. Edgar, Michael S. Kobor
    Pages 99-106

  9. Correcting for Sample Heterogeneity in Methylome-Wide Association Studies

    • James Y. Zou
    Pages 107-114

  10. Nano-MeDIP-seq Methylome Analysis Using Low DNA Concentrations

    • Lee M. Butcher, Stephan Beck
    Pages 115-138

  11. Bisulfite Conversion of DNA from Tissues, Cell Lines, Buffy Coat, FFPE Tissues, Microdissected Cells, Swabs, Sputum, Aspirates, Lavages, Effusions, Plasma, Serum, and Urine

    • Maria Jung, Barbara Uhl, Glen Kristiansen, Dimo Dietrich
    Pages 139-159

  12. Analysis of Imprinted Gene Regulation

    • David A. Skaar, Randy L. Jirtle
    Pages 161-183

  13. Statistical Methods for Methylation Data

    • Graham W. Horgan, Sok-Peng Chua
    Pages 185-203

  14. Back Matter

    Pages 205-206
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Keywords

About this book

This detailed volume focuses on population epigenetics, which is of increasing interest to policy makers searching for explanations for complex epidemiological observations and conceptual models on which to base interventions. With a concentration on DNA methylation, the emphasis is on the most commonly studied epigenetic phenomenon that is most amenable to further study. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Population Epigenetics: Methods and Protocols aims to aid population researchers in exploiting the latest insights into epigenetics to improve our understanding of the factors that influence human health and longevity.

Editors and Affiliations

  • Rowett Institute of Nutrition and Health, University of Aberdeen, Bucksburn, United Kingdom

    Paul Haggarty

  • Rowett Institute of Nutrition and Health, University of Aberdeen , Bucksburn, United Kingdom

    Kristina Harrison

Bibliographic Information

  • Book Title: Population Epigenetics

  • Book Subtitle: Methods and Protocols

  • Editors: Paul Haggarty, Kristina Harrison

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6903-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2017

  • Hardcover ISBN: 978-1-4939-6901-2Published: 17 February 2017

  • Softcover ISBN: 978-1-4939-8333-9Published: 12 July 2018

  • eBook ISBN: 978-1-4939-6903-6Published: 15 February 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 206

  • Topics: Human Genetics

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