Skip to main content
SpringerLink
Log in
Book cover

The Immune Synapse

Methods and Protocols

  • Book
  • © 2017

Overview

Editors:
  1. Cosima T. Baldari

    1. Department of Life sciences, University of Siena, Siena, Italy

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Michael L. Dustin

    1. University of Oxford, Kennedy Institute of Rheumatology, Headington, Oxford, United Kingdom

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1584)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 199.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 249.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (36 protocols)

  1. Front Matter

    Pages i-xiii
    Download chapter PDF

  2. The Immune Synapse: Past, Present, and Future

    • Michael L. Dustin, Cosima T. Baldari
    Pages 1-5

  3. Analyzing Actin Dynamics at the Immunological Synapse

    • Katarzyna I. Jankowska, Janis K. Burkhardt
    Pages 7-29

  4. Analysis of Microtubules and Microtubule-Organizing Center at the Immune Synapse

    • Noelia Blas-Rus, Eugenio Bustos-Morán, Francisco Sánchez-Madrid, Noa B. Martín-Cófreces
    Pages 31-49

  5. Analyzing the Dynamics of Signaling Microclusters

    • Akiko Hashimoto-Tane, Tadashi Yokosuka, Takashi Saito
    Pages 51-64

  6. Reconstitution of TCR Signaling Using Supported Lipid Bilayers

    • Xiaolei Su, Jonathon A. Ditlev, Michael K. Rosen, Ronald D. Vale
    Pages 65-76

  7. Plasma Membrane Sheets for Studies of B Cell Antigen Internalization from Immune Synapses

    • Carla R. Nowosad, Pavel Tolar
    Pages 77-88

  8. Studying the Dynamics of TCR Internalization at the Immune Synapse

    • Enrique Calleja, Balbino Alarcón, Clara L. Oeste
    Pages 89-99

  9. T Cell Receptor Activation of NF-κB in Effector T Cells: Visualizing Signaling Events Within and Beyond the Cytoplasmic Domain of the Immunological Synapse

    • Maria K. Traver, Suman Paul, Brian C. Schaefer
    Pages 101-127

  10. Imaging Vesicular Traffic at the Immune Synapse

    • Jérôme Bouchet, Iratxe del Río-Iñiguez, Andrés Alcover
    Pages 129-142

  11. Analysis of TCR/CD3 Recycling at the Immune Synapse

    • Laura Patrussi, Cosima T. Baldari
    Pages 143-155

  12. Simultaneous Membrane Capacitance Measurements and TIRF Microscopy to Study Granule Trafficking at Immune Synapses

    • Marwa Sleiman, David R. Stevens, Jens Rettig
    Pages 157-169

  13. Mathematical Modeling of Synaptic Patterns

    • Anastasios Siokis, Philippe A. Robert, Michael Meyer-Hermann
    Pages 171-182

  14. Super-resolution Analysis of TCR-Dependent Signaling: Single-Molecule Localization Microscopy

    • Valarie A. Barr, Jason Yi, Lawrence E. Samelson
    Pages 183-206

  15. Förster Resonance Energy Transfer to Study TCR-pMHC Interactions in the Immunological Synapse

    • Gerhard J. Schütz, Johannes B. Huppa
    Pages 207-229

  16. Two-Dimensional Analysis of Cross-Junctional Molecular Interaction by Force Probes

    • Lining Ju, Yunfeng Chen, Muaz Nik Rushdi, Wei Chen, Cheng Zhu
    Pages 231-258

  17. Studying Dynamic Plasma Membrane Binding of TCR-CD3 Chains During Immunological Synapse Formation Using Donor-Quenching FRET and FLIM-FRET

    • Etienne Gagnon, Audrey Connolly, Jessica Dobbins, Kai W. Wucherpfennig
    Pages 259-289

  18. Revealing the Role of Microscale Architecture in Immune Synapse Function Through Surface Micropatterning

    • Joung-Hyun Lee, Lance C. Kam
    Pages 291-306

  19. Spatial Control of Biological Ligands on Surfaces Applied to T Cell Activation

    • Haogang Cai, David Depoil, James Muller, Michael P. Sheetz, Michael L. Dustin, Shalom J. Wind
    Pages 307-331

  20. Probing Synaptic Biomechanics Using Micropillar Arrays

    • Weiyang Jin, Charles T. Black, Lance C. Kam, Morgan Huse
    Pages 333-346
Back to top

Keywords

About this book

This volume provides all the essential protocols that are currently used to study the immune synapse. Chapters in The Immune Synapse: Methods and Protocols cover methods for the study of the dynamics of immune synapse assembly, traffic at the immune synapse, new high resolution imaging, biophysical and computational methods for the study of the immune synapse, effector immune synapses, B cell, NK and mast cell immune synapses, and immune interactions in vivo. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, The Immune Synapse: Methods and Protocols will be of interest to immunologists and, at a more general level, to cell biologists, biophysicists and computational biologists.

Editors and Affiliations

  • Department of Life sciences, University of Siena, Siena, Italy

    Cosima T. Baldari

  • University of Oxford, Kennedy Institute of Rheumatology, Headington, Oxford, United Kingdom

    Michael L. Dustin

About the editors





Bibliographic Information

  • Book Title: The Immune Synapse

  • Book Subtitle: Methods and Protocols

  • Editors: Cosima T. Baldari, Michael L. Dustin

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6881-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • Hardcover ISBN: 978-1-4939-6879-4Published: 05 March 2017

  • Softcover ISBN: 978-1-4939-8327-8Published: 20 July 2018

  • eBook ISBN: 978-1-4939-6881-7Published: 01 March 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 588

  • Number of Illustrations: 25 b/w illustrations, 89 illustrations in colour

  • Topics: Immunology

Publish with us

Policies and ethics

Back to top

Search

Navigation