Chemical and Synthetic Approaches in Membrane Biology

1. Front Matter

   Pages i-xi

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2. Lipid-Coated Gold Nanoparticles as Probes for Membrane Binding

   • Desmond J. Hamilton, Yuheng Cai, Rupinder Kaur, Grant W. Marquart, Marilyn R. Mackiewicz, Scott M. Reed

   Pages 1-16

3. Delving into Lipid-Driven Endocytic Mechanisms Using Biomimetic Membranes

   • Josef Madl, Sarah Villringer, Winfried Römer

   Pages 17-36

4. Lipid Bilayer Patterns Fabrication by One-Photon Lithography

   • M. Florencia Sánchez, Dolores C. Carrer

   Pages 37-48

5. Approaches for Preparation and Biophysical Characterization of Transmembrane β-Barrels

   • Bharat Ramasubramanian Iyer, Ankit Gupta, Radhakrishnan Mahalakshmi

   Pages 49-116

6. Probing the Dynamics of Plasma Membrane Glutamate Transporters in Real Time by Total Internal Fluorescence Reflection Microscopy

   • Eliana S. Di Cairano, Paola Marciani, Stefania Moretti, Carla Perego

   Pages 117-139

7. Fluorescence-Based Screening of Membrane Proteins for Structural Studies

   • Jyoti Kumari, Surbhi Dhingra, Janesh Kumar

   Pages 141-155

8. Single-Molecule Localization Super-Resolution Microscopy of Synaptic Proteins

   • Francisco J. Barrantes

   Pages 157-198

9. Chemical Methods for Monitoring Protein Fatty Acylation

   • Xiaoqiu Yuan, Howard C. Hang

   Pages 199-217

10. Quantum Dot Toolbox in Membrane Neurotransmitter Transporter Research

    • Lucas B. Thal, Danielle M. Bailey, Oleg Kovtun, Sandra J. Rosenthal

    Pages 219-230

11. Generation of Synthetic Antibody Fragments to Detergent Solubilized Membrane Proteins

    • Serdar Uysal, Anthony Kossiakoff

    Pages 231-243

12. Pulse-Chase Covalent Labeling Technique for Monitoring GPCR Endocytosis

    • Hidetoshi Kumagai, Yuichi Ikeda

    Pages 245-252

13. Sample Preparation of Rhodopsins in the E. coli Membrane for In Situ Magic Angle Spinning Solid-State Nuclear Magnetic Resonance Studies

    • Meaghan E. Ward, Vladimir Ladizhansky, Leonid S. Brown

    Pages 253-267

14. Back Matter

    Pages 269-270

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This practical volume brings together a broad range of topics related to membrane biology research with particular emphasis on novel approaches, technology platforms, and emerging tools in this area of study. Beginning with chapters pertaining to artificial or designer membrane mimetics that can be utilized for in vitro studies of membrane-lipid interactions, it then continues by covering the approaches and methodologies to directly investigate membrane protein structure, localization, and dynamics.  Written for the Springer Protocols Handbooks series, this collection focuses on in-depth, hands-on protocols from experts in the field. 

Authoritative and cutting-edge, Chemical and Synthetic Approaches in Membrane Biology serves as an ideal guide to researchers investigating the vital properties of cellular membranes.

• Membrane protein structure
• Membrane protein dynamics
• Drug targets
• Novel therapeutics
• Lipid membranes
• Lipid-protein interactions
• Mimetics

• Indian Institute of Technology, Kanpur, India

  Arun K. Shukla

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