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Mycotoxigenic Fungi

Methods and Protocols

  • Book
  • © 2017

Overview

Editors:
  1. Antonio Moretti

    1. Institute of Sciences of Food Production National Research Council, Bari, Italy

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    You can also search for this editor in PubMed   Google Scholar

  2. Antonia Susca

    1. Institute of Sciences of Food Production National Research Council, Bari, Italy

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols for the study of the mycotoxigenic fungi
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1542)

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Table of contents (24 protocols)

  1. Front Matter

    Pages i-xi
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  2. Fungal Genera and Species of Major Significance and Their Associated Mycotoxins

    1. Front Matter

      Pages 1-1
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    2. Mycotoxins: An Underhand Food Problem

      • Antonio Moretti, Antonio F. Logrieco, Antonia Susca
      Pages 3-12

    3. Alternaria Species and Their Associated Mycotoxins

      • Virginia Elena Fernández Pinto, Andrea Patriarca
      Pages 13-32

    4. Aspergillus Species and Their Associated Mycotoxins

      • Giancarlo Perrone, Antonia Gallo
      Pages 33-49

    5. Fusarium Species and Their Associated Mycotoxins

      • Gary P. Munkvold
      Pages 51-106

    6. Penicillium Species and Their Associated Mycotoxins

      • Giancarlo Perrone, Antonia Susca
      Pages 107-119

  3. Polymerase Chain Reaction (PCR)-Based Methods for Detection and Identification of Mycotoxigenic Fungi

    1. Front Matter

      Pages 121-121
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    2. Targeting Conserved Genes in Alternaria Species

      • Miguel Ángel Pavón, Inés María López-Calleja, Isabel González, Rosario Martín, Teresa García
      Pages 123-129

    3. Targeting Conserved Genes in Aspergillus Species

      • Sándor Kocsubé, János Varga
      Pages 131-140

    4. Targeting Conserved Genes in Fusarium Species

      • Jéssica Gil-Serna, Belén Patiño, Miguel Jurado, Salvador Mirete, Covadonga Vázquez, M. Teresa González-Jaén
      Pages 141-147

    5. Targeting Conserved Genes in Penicillium Species

      • Stephen W. Peterson
      Pages 149-157

    6. Targeting Aflatoxin Biosynthetic Genes

      • Ali Y. Srour, Ahmad M. Fakhoury, Robert L. Brown
      Pages 159-171

    7. Targeting Trichothecene Biosynthetic Genes

      • Songhong Wei, Theo van der Lee, Els Verstappen, Marga van Gent, Cees Waalwijk
      Pages 173-189

    8. Targeting Ochratoxin Biosynthetic Genes

      • Antonia Gallo, Giancarlo Perrone
      Pages 191-200

    9. Targeting Fumonisin Biosynthetic Genes

      • Robert H. Proctor, Martha M. Vaughan
      Pages 201-214

    10. Targeting Other Mycotoxin Biosynthetic Genes

      • María J. Andrade, Mar Rodríguez, Juan J. Córdoba, Alicia Rodríguez
      Pages 215-235

    11. Evaluating Aflatoxin Gene Expression in Aspergillus Section Flavi

      • Paula Cristina Azevedo Rodrigues, Jéssica Gil-Serna, M. Teresa González-Jaén
      Pages 237-247

    12. Evaluating Fumonisin Gene Expression in Fusarium verticillioides

      • Valeria Scala, Ivan Visentin, Francesca Cardinale
      Pages 249-257

  4. Polymerase Chain Reaction (PCR)-Based Methods for Multiplex Detection of Mycotoxigenic Fungi

    1. Front Matter

      Pages 259-259
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Keywords

About this book

This thorough volume explores the possibility of detecting and identifying toxigenic fungi, able to produce secondary metabolites known as mycotoxins, which cause severe health problems in humans and animals after exposure to contaminated food and feed, having a broad range of toxic effects, including carcinogenicity, neurotoxicity, and reproductive and developmental toxicity. Beginning with a section on fungal genera and species of major significance along with their associated mycotoxins, the book continues with sections on Polymerase Chain Reaction (PCR)-based methods for the detection and identification of mycotoxigenic fungi, PCR-based methods for multiplex detection of mycotoxigenic fungi, as well as sections on combined approaches and new methodologies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratoryprotocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Mycotoxigenic Fungi: Methods and Protocols will aid researchers working in this vital field to provide insight into possible actions to reduce mycotoxin contamination of crop plants and the food/feed byproducts.

Editors and Affiliations

  • Institute of Sciences of Food Production National Research Council, Bari, Italy

    Antonio Moretti, Antonia Susca

Bibliographic Information

  • Book Title: Mycotoxigenic Fungi

  • Book Subtitle: Methods and Protocols

  • Editors: Antonio Moretti, Antonia Susca

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6707-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • Hardcover ISBN: 978-1-4939-6705-6Published: 06 December 2016

  • Softcover ISBN: 978-1-4939-8279-0Published: 07 July 2018

  • eBook ISBN: 978-1-4939-6707-0Published: 05 December 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 383

  • Number of Illustrations: 28 b/w illustrations, 17 illustrations in colour

  • Topics: Microbiology

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