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Oat

Methods and Protocols

  • Book
  • © 2017

Overview

Editors:
  1. Sebastian Gasparis

    1. Department of Functional Genomics, Plant Breeding & Acclimatization Inst. National Research Institute, Blonie, Poland

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1536)

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Table of contents (18 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. Cytological Methods

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Fluorescence In Situ Hybridization in Oat

      • Eva Wegel
      Pages 3-21

    3. Oat Doubled Haploids Following Maize Pollination

      • Philip A. Davies, Parminder K. Sidhu
      Pages 23-30

    4. Wide Hybridization Between Oat and Pearl Millet

      • Takayoshi Ishii
      Pages 31-42

    5. Protocol for Producing Synthetic Polyploid Oats

      • Toshinobu Morikawa
      Pages 43-52

  3. In Vitro Cultures and Genetic Transformation

    1. Front Matter

      Pages 53-53
      Download chapter PDF

    2. Manipulation of Oat Protoplasts for Transient Expression Assays

      • Robyn Roberts, Jincan Zhang, Nicole Mihelich, Danielle Savino, Aurélie M. Rakotondrafara
      Pages 55-70

    3. Oat Anther Culture and Use of DH-Lines for Genetic Mapping

      • Elina Kiviharju, Sirpa Moisander, Pirjo Tanhuanpää
      Pages 71-93

    4. Agrobacterium-Mediated Transformation of Leaf Base Segments

      • Sebastian Gasparis
      Pages 95-111

  4. Quality Assessment

    1. Front Matter

      Pages 113-113
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    2. Chromatographic Methods to Evaluate Nutritional Quality in Oat

      • Gracia Montilla-Bascón, Corey D. Broeckling, Owen A. Hoekenga, Elena Prats, Mark Sorrells, Julio Isidro-Sánchez
      Pages 115-125

    3. Determination of T-2 and HT-2 Toxins in Oats and Oat-Based Breakfast Cereals by Liquid-Chromatography Tandem Mass Spectrometry

      • Veronica M. T. Lattanzio, Michelangelo Pascale
      Pages 127-136

    4. Multiplex Dipstick Immunoassay for Semiquantitative Determination of Fusarium Mycotoxins in Oat

      • Veronica M. T. Lattanzio, Noan Nivarlet
      Pages 137-142

    5. Microarray-Based Immunoassay for Parallel Quantification of Multiple Mycotoxins in Oat

      • Susanna Oswald, Richard Dietrich, Erwin Märtlbauer, Reinhard Niessner, Dietmar Knopp
      Pages 143-156

  5. Genomic Studies

    1. Front Matter

      Pages 157-157
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    2. M13-Tailed Simple Sequence Repeat (SSR) Markers in Studies of Genetic Diversity and Population Structure of Common Oat Germplasm

      • Agnieszka OnyÅ›k, Maja Boczkowska
      Pages 159-168

    3. Genotyping-by-Sequencing and Its Application to Oat Genomic Research

      • Yong-Bi Fu, Mo-Hua Yang
      Pages 169-187

    4. Genome-Wide Association Analysis Using R

      • Julio Isidro-Sánchez, Deniz Akdemir, Gracia Montilla-Bascón
      Pages 189-207

    5. De Novo Transcriptome Assembly in Polyploid Species

      • Juan J. Gutierrez-Gonzalez, David F. Garvin
      Pages 209-221
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Keywords

About this book

The volume provides detailed protocols that have been developed or modified exclusively for the study of oat. The topics discussed in this book are a selection of various molecular biology and biotechnology methods, such as the application of molecular markers for polymorphism analyses and cytological manipulations, the production of synthetic polyploids, and in vitro cultures and genetic modifications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Oat: Methods and Protocols is a useful resource in the development of new research approaches toward organizing the oat genome and the identification of new and useful traits for further improvements of this exceptional crop.

Editors and Affiliations

  • Department of Functional Genomics, Plant Breeding & Acclimatization Inst. National Research Institute, Blonie, Poland

    Sebastian Gasparis

Bibliographic Information

  • Book Title: Oat

  • Book Subtitle: Methods and Protocols

  • Editors: Sebastian Gasparis

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6682-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • Hardcover ISBN: 978-1-4939-6680-6Published: 29 January 2017

  • Softcover ISBN: 978-1-4939-8271-4Published: 15 July 2018

  • eBook ISBN: 978-1-4939-6682-0Published: 25 January 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 272

  • Number of Illustrations: 13 b/w illustrations, 25 illustrations in colour

  • Topics: Plant Sciences

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