Cardiac Gene Therapy

1. Front Matter

   Pages i-xi

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2. Overview

   1. Front Matter

      Pages 1-1

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   2. Current Methods in Cardiac Gene Therapy: Overview

      • Kiyotake Ishikawa, Roger J. Hajjar

      Pages 3-14

3. Genetic Modification

   1. Front Matter

      Pages 15-15

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   2. Silencing Genes in the Heart

      • Henry Fechner, Roland Vetter, Jens Kurreck, Wolfgang Poller

      Pages 17-39

   3. Generation of Efficient miRNA Inhibitors Using Tough Decoy Constructs

      • Jimeen Yoo, Roger J. Hajjar, Dongtak Jeong

      Pages 41-53

   4. Efficient Genome Editing in Induced Pluripotent Stem Cells with Engineered Nucleases In Vitro

      • Vittavat Termglinchan, Timon Seeger, Caressa Chen, Joseph C. Wu, Ioannis Karakikes

      Pages 55-68

   5. Direct Cardiac Reprogramming as a Novel Therapeutic Strategy for Treatment of Myocardial Infarction

      • Hong Ma, Li Wang, Jiandong Liu, Li Qian

      Pages 69-88

4. Gene Delivery Vectors

   1. Front Matter

      Pages 89-89

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   2. Production and Characterization of Vectors Based on the Cardiotropic AAV Serotype 9

      • Erik Kohlbrenner, Thomas Weber

      Pages 91-107

   3. Cell-Based Measurement of Neutralizing Antibodies Against Adeno-Associated Virus (AAV)

      • Andreas Jungmann, Oliver Müller, Kleopatra Rapti

      Pages 109-126

   4. Synthesis of Modified mRNA for Myocardial Delivery

      • Jason Kondrat, Nishat Sultana, Lior Zangi

      Pages 127-138

   5. Exosomes-Based Gene Therapy for MicroRNA Delivery

      • Prabhu Mathiyalagan, Susmita Sahoo

      Pages 139-152

   6. Lipidoid mRNA Nanoparticles for Myocardial Delivery in Rodents

      • Irene C. Turnbull, Ahmed A. Eltoukhy, Daniel G. Anderson, Kevin D. Costa

      Pages 153-166

5. Gene Delivery Methods

   1. Front Matter

      Pages 167-167

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   2. Gene Transfer in Isolated Adult Cardiomyocytes

      • Kjetil Hodne, David B. Lipsett, William E. Louch

      Pages 169-182

   3. Gene Transfer in Cardiomyocytes Derived from ES and iPS Cells

      • Francesca Stillitano, Ioannis Karakikes, Roger J. Hajjar

      Pages 183-193

   4. Gene Transfer to Rodent Hearts In Vivo

      • Federica del Monte, Kiyotake Ishikawa, Roger J. Hajjar

      Pages 195-204

   5. Ultrasound-Targeted Microbubble Destruction for Cardiac Gene Delivery

      • Shuyuan Chen, Paul A. Grayburn

      Pages 205-218

   6. A Needleless Liquid Jet Injection Delivery Approach for Cardiac Gene Therapy

      • Anthony S. Fargnoli, Michael G. Katz, Charles R. Bridges

      Pages 219-226

This detailed book provides methodological information on cardiac gene delivery, from classic to state-of-the-art technologies and techniques. Efficient, cardiac-specific, and safe vectors, as well as refined vector delivery methods, are key for successful cardiac gene transfer and eventually for improving patients’ outcomes. Newer vectors and more efficient vector delivery methods have the potential to dramatically improve gene transduction efficacy, while novel gene manipulation techniques enforce the therapeutic power and broaden disease targets. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Cardiac Gene Therapy: Methods and Protocols serves as a valuable tool for molecular biologists and physiologists in the cardiology field conducting cardiac gene transfer research, which will ultimately lead to further advancements in the vital field.

• Vector technologies
• Cardiovascular disease treatment
• Cardiac gene transfer
• Vector delivery methods
• Gene transduction
• Cardiology
• Disease targets

• Cardiovascular Research Center, Icahn School of Medicine at Mount Sinai, New York, USA

  Kiyotake Ishikawa

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