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Plant Hormones

Methods and Protocols

  • Book
  • © 2017

Overview

Editors:
  1. Jürgen Kleine-Vehn

    1. IAGZ, Universität für Bodenkultur Wien, Vienna, Austria

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    You can also search for this editor in PubMed   Google Scholar

  2. Michael Sauer

    1. Institut für Biochemie und Biologie, Universität Potsdam, Potsdam, Germany

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1497)

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Table of contents (22 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Real-Time Analysis of the Apical Hook Development

    • Qiang Zhu, Petra Žádníková, Dajo Smet, Dominique Van Der Straeten, Eva Benková
    Pages 1-8

  3. Grafting with Arabidopsis thaliana

    • Charles W. Melnyk
    Pages 9-18

  4. Tips and Tricks for Exogenous Application of Synthetic Post-translationally Modified Peptides to Plants

    • Nathan Czyzewicz, Elisabeth Stes, Ive De Smet
    Pages 19-28

  5. Assaying Germination and Seedling Responses of Arabidopsis to Karrikins

    • Mark T. Waters, Gavin R. Flematti, Steven M. Smith
    Pages 29-36

  6. Low-Cost Microprocessor-Controlled Rotating Stage for Medium-Throughput Time-Lapse Plant Phenotyping

    • Francis Barbez, Jürgen Kleine-Vehn, Elke Barbez
    Pages 37-45

  7. Genome-Wide Association Mapping of Root Traits in the Context of Plant Hormone Research

    • Daniela Ristova, Wolfgang Busch
    Pages 47-55

  8. High-Throughput Scoring of Seed Germination

    • Wilco Ligterink, Henk W. M. Hilhorst
    Pages 57-72

  9. Histochemical Staining of β-Glucuronidase and Its Spatial Quantification

    • Chloé Béziat, Jürgen Kleine-Vehn, Elena Feraru
    Pages 73-80

  10. Imaging TCSn::GFP, a Synthetic Cytokinin Reporter, in Arabidopsis thaliana

    • Jingchun Liu, Bruno Müller
    Pages 81-90

  11. Highlighting Gibberellins Accumulation Sites in Arabidopsis thaliana Root Using Fluorescently Labeled Gibberellins

    • Hilla Schayek, Eilon Shani, Roy Weinstain
    Pages 91-97

  12. In Silico Methods for Cell Annotation, Quantification of Gene Expression, and Cell Geometry at Single-Cell Resolution Using 3DCellAtlas

    • Petra Stamm, Soeren Strauss, Thomas D. Montenegro-Johnson, Richard Smith, George W. Bassel
    Pages 99-123

  13. Analyzing Cell Wall Elasticity After Hormone Treatment: An Example Using Tobacco BY-2 Cells and Auxin

    • Siobhan A. Braybrook
    Pages 125-133

  14. FRET-FLIM for Visualizing and Quantifying Protein Interactions in Live Plant Cells

    • Alejandra Freire Rios, Tatyana Radoeva, Bert De Rybel, Dolf Weijers, Jan Willem Borst
    Pages 135-146

  15. In Vivo Identification of Plant Protein Complexes Using IP-MS/MS

    • Jos R. Wendrich, Sjef Boeren, Barbara K. Möller, Dolf Weijers, Bert De Rybel
    Pages 147-158

  16. Assaying Auxin Receptor Activity Using SPR Assays with F-Box Proteins and Aux/IAA Degrons

    • Mussa Quareshy, Veselina Uzunova, Justyna M. Prusinska, Richard M. Napier
    Pages 159-191

  17. Studying Transcription Factor Binding to Specific Genomic Loci by Chromatin Immunoprecipitation (ChIP)

    • S. Vinod Kumar, Doris Lucyshyn
    Pages 193-203

  18. Hormone Receptor Glycosylation

    • Ulrike Vavra, Christiane Veit, Richard Strasser
    Pages 205-220

  19. Highly Sensitive Salicylic Acid Quantification in Milligram Amounts of Plant Tissue

    • Víctor Carrasco Loba, Stephan Pollmann
    Pages 221-229

  20. High-Resolution Cell-Type Specific Analysis of Cytokinins in Sorted Root Cell Populations of Arabidopsis thaliana

    • Ondřej Novák, Ioanna Antoniadi, Karin Ljung
    Pages 231-248
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Keywords

About this book

This volume aims to present a representative cross-section of modern experimental approaches relevant to Plant Hormone Biology, ranging from relatively simple physiological to highly sophisticated methods. Chapters describe physiological, developmental, microscopy-based techniques, measure hormone contents, and heterologous systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Plant Hormones:Methods and Protocols, Third Edition aims to provide researchers with useful methods to advance their research.

Editors and Affiliations

  • IAGZ, Universität für Bodenkultur Wien, Vienna, Austria

    Jürgen Kleine-Vehn

  • Institut für Biochemie und Biologie, Universität Potsdam, Potsdam, Germany

    Michael Sauer

Bibliographic Information

  • Book Title: Plant Hormones

  • Book Subtitle: Methods and Protocols

  • Editors: Jürgen Kleine-Vehn, Michael Sauer

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6469-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2017

  • Hardcover ISBN: 978-1-4939-6467-3Published: 18 November 2016

  • Softcover ISBN: 978-1-4939-8210-3Published: 08 June 2018

  • eBook ISBN: 978-1-4939-6469-7Published: 16 November 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 3

  • Number of Pages: XI, 288

  • Number of Illustrations: 11 b/w illustrations, 106 illustrations in colour

  • Topics: Plant Sciences

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