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Polycomb Group Proteins

Methods and Protocols

  • Book
  • © 2016

Overview

Editors:
  1. Chiara Lanzuolo

    1. IBCN-CNR, Institute of Cell Biology and Neurobiology and Istituto Nazionale di Genetica Molecolare “Romeo ed Enrica Invernizzi”, Rome, Italy

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    You can also search for this editor in PubMed   Google Scholar

  2. Beatrice Bodega

    1. Istituto Nazionale di Genetica Molecolare “Romeo ed Enrica Invernizzi”, Milan, Italy

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1480)

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Table of contents (28 protocols)

  1. Front Matter

    Pages i-xv
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  2. Part I

    1. Front Matter

      Pages 1-1
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    2. Mapping the Function of Polycomb Proteins

      • Diego Pasini
      Pages 3-6

    3. Chromatin Immunoprecipitation

      • Laura Wiehle, Achim Breiling
      Pages 7-21

    4. Chromatin Preparation and Chromatin Immuno-precipitation from Drosophila Embryos

      • Eva Löser, Daniel Latreille, Nicola Iovino
      Pages 23-36

    5. ChIP-seq Data Processing for PcG Proteins and Associated Histone Modifications

      • Ozren Bogdanovic´, Simon J. van Heeringen
      Pages 37-53

    6. Analysis of Single-Locus Replication Timing in Asynchronous Cycling Cells

      • Lo Sardo Federica
      Pages 55-65

  3. Part II

    1. Front Matter

      Pages 67-67
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    2. Noncoding RNA Interplay with the Genome

      • Davide Gabellini
      Pages 69-72

    3. RIP: RNA Immunoprecipitation

      • Miriam Gagliardi, Maria R. Matarazzo
      Pages 73-86

    4. Capture Hybridization Analysis of DNA Targets

      • Alec N. Sexton, Martin Machyna, Matthew D. Simon
      Pages 87-97

    5. Identification of RNA–Protein Interactions Through In Vitro RNA Pull-Down Assays

      • Claire Barnes, Aditi Kanhere
      Pages 99-113

    6. Understanding RNA-Chromatin Interactions Using Chromatin Isolation by RNA Purification (ChIRP)

      • Ci Chu, Howard Y. Chang
      Pages 115-123

    7. Analysis RNA-seq and Noncoding RNA

      • Alberto Arrigoni, Valeria Ranzani, Grazisa Rossetti, Ilaria Panzeri, Sergio Abrignani, Raoul J. P. Bonnal et al.
      Pages 125-135

  4. Part III

    1. Front Matter

      Pages 137-137
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    2. The Dynamics of Polycomb Complexes

      • Daniela Palacios
      Pages 139-142

    3. Fluorescence Resonance Energy Transfer Microscopy for Measuring Chromatin Complex Structure and Dynamics

      • Alessandro Cherubini, Alessio Zippo
      Pages 143-152

    4. Analysis of Endogenous Protein Interactions of Polycomb Group of Proteins in Mouse Embryonic Stem Cells

      • Lluis Morey, Luciano Di Croce
      Pages 153-165

    5. Determination of Polycomb Group of Protein Compartmentalization Through Chromatin Fractionation Procedure

      • Federica Marasca, Fabrizia Marullo, Chiara Lanzuolo
      Pages 167-180

    6. An Automatic Segmentation Method Combining an Active Contour Model and a Classification Technique for Detecting Polycomb-group Proteinsin High-Throughput Microscopy Images

      • Francesco Gregoretti, Elisa Cesarini, Chiara Lanzuolo, Gennaro Oliva, Laura Antonelli
      Pages 181-197
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Keywords

About this book

This volume presents the most recent technologies used in the Polycomb Group Proteins (PcG) field. Chapters detail state-of-the-art methods, creating a unique and comprehensive reference source for investigating Polycomb function in the nucleus. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Polycomb Group Proteins: Method and Protocols aims to ensure successful results in the further study of this vital field.

Editors and Affiliations

  • IBCN-CNR, Institute of Cell Biology and Neurobiology and Istituto Nazionale di Genetica Molecolare “Romeo ed Enrica Invernizzi”, Rome, Italy

    Chiara Lanzuolo

  • Istituto Nazionale di Genetica Molecolare “Romeo ed Enrica Invernizzi”, Milan, Italy

    Beatrice Bodega

Bibliographic Information

  • Book Title: Polycomb Group Proteins

  • Book Subtitle: Methods and Protocols

  • Editors: Chiara Lanzuolo, Beatrice Bodega

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6380-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-6378-2Published: 23 September 2016

  • Softcover ISBN: 978-1-4939-8181-6Published: 27 June 2018

  • eBook ISBN: 978-1-4939-6380-5Published: 22 September 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XV, 325

  • Number of Illustrations: 17 b/w illustrations, 23 illustrations in colour

  • Topics: Protein Science

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