Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 1475)
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Table of contents (21 protocols)
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Front Matter
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Procedures to Study Protein SUMOylation In Vitro
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Front Matter
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Analysis of Protein SUMOylation Using Cell Lines
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Front Matter
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Procedures to Study Protein SUMOylation In Vivo
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Front Matter
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Keywords
About this book
This volume explores various methodologies to study biochemical, molecular, and cellular biology aspects of some processes regulated by protein SUMOylation. SUMO: Methods and Protocols is organized into four parts, and starts with an historical overview on protein SUMOylation and a presentation of the methods included in the book. The first part also includes a review on chromatin regulation by dynamic SUMO modifications. The second part focuses on in vitro techniques, including biochemical methods to study mechanistic aspects of protein SUMOylation. The third part includes protocols to be used with cell cultures, which often are the first approaches used in most laboratories. The final part includes methodologies adapted for the analysis in vivo using distinct model organisms. Written in the highly successful Methods in Molecular Biology series format, chapters include a brief introduction to the subject, a list of necessary materials and reagents, a step-by-step reproducible laboratory protocol ending with a Notes section on troubleshooting tips, and tips and strategies to avoid known pitfalls.
Unique and cutting-edge, SUMO: Methods and Protocols provides a comprehensive source of protocols for specialists and researchers not familiar with this vital system.
Editors and Affiliations
Bibliographic Information
Book Title: SUMO
Book Subtitle: Methods and Protocols
Editors: Manuel S. Rodriguez
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-6358-4
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2016
Hardcover ISBN: 978-1-4939-6356-0Published: 08 September 2016
Softcover ISBN: 978-1-4939-8175-5Published: 21 April 2018
eBook ISBN: 978-1-4939-6358-4Published: 08 September 2016
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XI, 306
Number of Illustrations: 20 b/w illustrations, 34 illustrations in colour
Topics: Biochemistry, general