Western Blotting

1. Front Matter

   Pages i-xiv

   PDF

2. The Early Days of Blotting

   • Edwin Southern

   Pages 1-3

3. Origins of Protein Blotting

   • Harry Towbin

   Pages 5-7

4. Western Blotting: Remembrance of Things Past

   • W. Neal Burnette

   Pages 9-12

5. Simian Virus 40 and Protein Transfer

   • Jaime Renart

   Pages 13-16

6. Western Blotting: An Introduction

   • Biji T. Kurien, R. Hal Scofield

   Pages 17-30

7. From Little Helpers to Automation

   • Biji T. Kurien, Harry Towbin

   Pages 31-40

8. Spectrophotometric Methods to Determine Protein Concentration

   • J. P. Dean Goldring

   Pages 41-47

9. Solubilization of Proteins: The Importance of Lysis Buffer Choice

   • Mandy Peach, Noelle Marsh, Ewa I. Miskiewicz, Daniel J. MacPhee

   Pages 49-60

10. Simultaneous Immunoblotting Analysis with Activity Gel Electrophoresis and 2-D Gel Electrophoresis

    • Der-Yen Lee, Geen-Dong Chang

    Pages 61-72

11. Diffusion Blotting: A Rapid and Simple Method for Production of Multiple Blots from a Single Gel

    • Ingrid Olsen, Harald G. Wiker

    Pages 73-76

12. Multiple Immunoblots by Passive Diffusion of Proteins from a Single SDS-PAGE Gel

    • Biji T. Kurien, R. Hal Scofield

    Pages 77-86

13. Slice Blotting

    • Graeme Lowe

    Pages 87-92

14. Localizing Proteins by Tissue Printing

    • Rafael F. Pont-Lezica

    Pages 93-104

15. Dot-Immunobinding Assay (Dot-Iba)

    • Sumi Surendran, Annamma Mathai, Vishnampet Venkataraman Radhakrishnan

    Pages 105-108

16. Analysis of Antibody Clonotype by Affinity Immunoblotting

    • Biji T. Kurien, R. Hal Scofield

    Pages 109-117

17. Glycosaminoglycan Blotting and Detection After Electrophoresis Separation

    • Nicola Volpi, Francesca Maccari

    Pages 119-127

18. A Well-Based Reverse-Phase Protein Array of Formalin-Fixed Paraffin-Embedded Tissue

    • Joon-Yong Chung, Stephen M. Hewitt

    Pages 129-139

19. Quantitative Computerized Western Blotting in Detail

    • Dalit Talmi-Frank, Charles L. Jaffe, Gad Baneth

    Pages 141-150

20. Cationic Electrophoresis and Eastern Blotting

    • Engelbert Buxbaum

    Pages 151-163

This volume covers past and present western blot techniques, such as diffusion blotting, slice blotting, blotting of high and low molecular weight proteins, single cell blotting and automated blotting. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Thorough and cutting-edge, Western Blotting: Methods and Protocols will serve as an invaluable reference for those interested in further study into this fascinating field.

 

• Antigenic Peptides
• Centrifuge Blotting
• Diffusion Blotting
• Electroblotting
• Immunoprecipitation
• Protein
• Slice Blotting
• Western Immublotting

• Department of Medicine, University of Oklahoma, Arthritis and Clinical Immunology, Oklahoma Medical Research Foundation, Health Sciences Center, Department of Veterans, Oklahoma City, USA

  Biji T. Kurien

• Department of Medicine, Arthritis and Clinical Immunology, Oklahoma Medical Research Foundation, Health Sciences Center, Department of Veterans Affairs Medical Center, Oklahoma City, USA

  R. Hal Scofield

Biji Kurien, PhD Associate Professor of Research Oklahoma Medical Research Foundation (OMRF) Arthritis & Clinical Immunology Research Program 825 NE 13th St. MS24 Oklahoma City, OK 73104 Tel: (405) 271-8000 ext 35204 Email: biji-kurien@ouhsc.edu Robert H. Scofield, MD Arthritis & Clinical Immunology Research Program, Oklahoma Medical Research Foundation 825 N.E. 13th Street. MS 38 Oklahoma City, OK 73104 Phone: (405) 271-7061 Fax: (405) 271-7063 E-mail: Hal-Scofield@omrf.org

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