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  • © 2016

Estrogen Receptors

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1366)

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eBook USD 119.00
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Hardcover Book USD 169.99
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Table of contents (47 protocols)

  1. Front Matter

    Pages i-xvi
  2. Bioinformatics Analysis of Estrogen-Responsive Genes

    • Adam E. Handel
    Pages 29-39Open Access
  3. Chromatin Immunoprecipitation Assay to Identify Genomic Binding Sites of Regulatory Factors

    • Meike Wagner, Johannes Jung, Michael Koslowski, Özlem Türeci, Vijay K. Tiwari, Ugur Sahin
    Pages 53-65
  4. RNA-Seq Experiment and Data Analysis

    • Hanquan Liang, Erliang Zeng
    Pages 99-114
  5. DNA Microarray Analysis of Estrogen-Responsive Genes

    • Kathleen M. Eyster
    Pages 115-129
  6. Colocalization of Estrogen Receptors with the Fluorescent Tamoxifen Derivative, FLTX1, Analyzed by Confocal Microscopy

    • Araceli Morales, Raquel Marín, Jorge Marrero-Alonso, Alicia Boto, Mario Díaz
    Pages 163-173

About this book

This volume examines a wide array of vital technologies for advancing our understanding of the receptor-mediated actions of estrogen. The protocols in this book range from standard methods and vital laboratory workhorses, such as receptor binding assays and western blot, to newer technologies such as RNAseq and proximity ligation assay. Chapters also discuss protocols from a broad range of tissue types to demonstrate the variety of estrogen receptor effects. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Estrogen Receptors: Methods and Protocols is a detailed and helpful resource for scientists who are intrigued by the many facets of estrogen.

The chapter 'Bioinformatics Analysisof Estrogen-Responsive Genes' is open access under a CC BY 4.0 license. 

Editors and Affiliations

  • University of South Dakota Sanford School of Medicine, Vermillion, USA

    Kathleen M. Eyster

About the editor

Kathleen (Kathy) Eyster Professor of Physiology and Pharmacology Sanford School of Medicine of the University of South Dakota 605-677-5159

Bibliographic Information

  • Book Title: Estrogen Receptors

  • Book Subtitle: Methods and Protocols

  • Editors: Kathleen M. Eyster

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-3127-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-3126-2Published: 20 November 2015

  • Softcover ISBN: 978-1-4939-5006-5Published: 23 August 2016

  • eBook ISBN: 978-1-4939-3127-9Published: 19 November 2015

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVI, 576

  • Number of Illustrations: 41 b/w illustrations, 87 illustrations in colour

  • Topics: Biochemistry, general, Endocrinology

Buy it now

Buying options

eBook USD 119.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 159.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access