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Hox Genes

Methods and Protocols

  • Book
  • © 2014

Overview

Editors:
  1. Yacine Graba

    1. IBDML, Marseille, France

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    You can also search for this editor in PubMed   Google Scholar

  2. René Rezsohazy

    1. Université Catholique de Louvain, Louvain-la-Neuve, Belgium

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols involving Hox genes and Hox proteins
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice

Part of the book series: Methods in Molecular Biology (MIMB, volume 1196)

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Table of contents (21 protocols)

  1. Front Matter

    Pages i-xvii
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  2. Hox Genes: From Discovery to Functional Analysis

    1. Front Matter

      Pages 1-1
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    2. Discovery and Classification of Homeobox Genes in Animal Genomes

      • Ferdinand Marlétaz, Jordi Paps, Ignacio Maeso, Peter W. H. Holland
      Pages 3-18

    3. How to Study Hox Gene Expression and Function in Mammalian Oocytes and Early Embryos

      • Delphine Paul, Caroline Sauvegarde, René Rezsohazy, Isabelle Donnay
      Pages 19-36

    4. Genetic Lineage Tracing Analysis of Anterior Hox Expressing Cells

      • Brigitte Laforest, Nicolas Bertrand, Stéphane Zaffran
      Pages 37-48

    5. A Genetic Strategy to Obtain P-Gal4 Elements in the Drosophila Hox Genes

      • Luis de Navas, David Foronda, Delia del Saz, Ernesto Sánchez-Herrero
      Pages 49-57

    6. Hox Complex Analysis Through BAC Recombineering

      • Mark Parrish, Youngwook Ahn, Christof Nolte, Bony De Kumar, Robb Krumlauf
      Pages 59-87

    7. The Genetics of Murine Hox Loci: TAMERE, STRING, and PANTHERE to Engineer Chromosome Variants

      • Patrick Tschopp, Denis Duboule
      Pages 89-102

    8. Topological Organization of Drosophila Hox Genes Using DNA Fluorescent In Situ Hybridization

      • Frédéric Bantignies, Giacomo Cavalli
      Pages 103-120

    9. Mining the Cis-Regulatory Elements of Hox Clusters

      • Navneet Kaur Matharu, Rakesh K. Mishra
      Pages 121-131

    10. Functional Analysis of Hox Genes in Zebrafish

      • Franck Ladam, Charles G. Sagerström
      Pages 133-144

    11. Transgenesis in Non-model Organisms: The Case of Parhyale

      • Zacharias Kontarakis, Anastasios Pavlopoulos
      Pages 145-181

    12. Tissue Specific RNA Isolation in Drosophila Embryos: A Strategy to Analyze Context Dependent Transcriptome Landscapes Using FACS

      • Arnaud Defaye, Laurent Perrin
      Pages 183-195

    13. Hox Transcriptomics in Drosophila Embryos

      • Maria Polychronidou, Ingrid Lohmann
      Pages 197-208

  3. Hox Proteins: Mode of Action and Biomedical Applications

    1. Front Matter

      Pages 209-209
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    2. Measuring Hox-DNA Binding by Electrophoretic Mobility Shift Analysis

      • Kelly Churion, Ying Liu, Hao-Ching Hsiao, Kathleen S. Matthews, Sarah E. Bondos
      Pages 211-230

    3. Chromatin Immunoprecipitation and Chromatin Immunoprecipitation with Massively Parallel Sequencing on Mouse Embryonic Tissue

      • Shilu Amin, Nicoletta Bobola
      Pages 231-239

    4. ChIP for Hox Proteins from Drosophila Imaginal Discs

      • Pavan Agrawal, L. S. Shashidhara
      Pages 241-253

    5. SELEX-seq: A Method for Characterizing the Complete Repertoire of Binding Site Preferences for Transcription Factor Complexes

      • Todd R. Riley, Matthew Slattery, Namiko Abe, Chaitanya Rastogi, Dahong Liu, Richard S. Mann et al.
      Pages 255-278

    6. DamID as an Approach to Studying Long-Distance Chromatin Interactions

      • Fabienne Cléard, François Karch, Robert K. Maeda
      Pages 279-289
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Keywords

About this book

Hox Genes: Methods and Protocols explores techniques and methodologies which arose from or were successfully applied to the study of Hox genes and Hox proteins, at the intersection of experimental embryology, genetics, biochemistry, physiology, evolutionary biology and other life sciences. This detailed volume begins with a section on discovery and functional analysis of Hox genes and then it continues onward to discuss mode of action and biomedical applications of Hox proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Expert and practical, Hox Genes: Methods and Protocols serves as an ideal guide to researchers striving to move forward in this dynamic and exciting area of study.

Editors and Affiliations

  • IBDML, Marseille, France

    Yacine Graba

  • Université Catholique de Louvain, Louvain-la-Neuve, Belgium

    René Rezsohazy

Bibliographic Information

  • Book Title: Hox Genes

  • Book Subtitle: Methods and Protocols

  • Editors: Yacine Graba, René Rezsohazy

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-1242-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2014

  • Hardcover ISBN: 978-1-4939-1241-4Published: 24 August 2014

  • Softcover ISBN: 978-1-4939-4666-2Published: 22 September 2016

  • eBook ISBN: 978-1-4939-1242-1Published: 23 August 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVII, 373

  • Number of Illustrations: 22 b/w illustrations, 59 illustrations in colour

  • Topics: Human Genetics

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