Overview
- Includes cutting-edge methods and protocols for the study of pseudogenes and the avoidance of inadvertent detection
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1167)
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Table of contents (21 protocols)
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Introductory Chapters
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Methods to Identify Pseudogenes
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Methods to Detect Transcribed Pseudogenes of a Parental Gene of Interest
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High-Throughput Methods to Detect Transcribed Pseudogenes
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High-Throughput Methods to Detect Translated Pseudogenes
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Methods to Study Transcribed Pseudogenes
Keywords
About this book
Providing a list of methods useful both to those who wish to study pseudogenes and to those who actually want to avoid their inadvertent detection, Pseudogenes: Functions and Protocols explores techniques involving pseudogenic DNA, RNA, and peptides/proteins, once believed to lack any functionality, but now known to be involved in complex regulatory circuits. After a few introductory chapters that overview the functions so far attributed to pseudogenes, this thorough volume delves into methods for pseudogene identification, for the detection of pseudogene transcription and translation, and for the study of the functions of pseudogenic RNA and proteins, as well as methods to avoid pseudogene detection when the focus of the research is their highly homologous parental counterparts. As part of the highly successful Methods in Molecular Biology series, chapters feature the kind of detailed descriptions and implementation advice that ensures successful results in the lab.
Authoritative and practical, Pseudogenes: Functions and Protocols will contribute to the high interest of the scientific community toward pseudogenes, while stimulating the conception of pseudogene - centered research projects and providing experimental protocols that can facilitate their execution.
Editors and Affiliations
Bibliographic Information
Book Title: Pseudogenes
Book Subtitle: Functions and Protocols
Editors: Laura Poliseno
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-0835-6
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2014
Hardcover ISBN: 978-1-4939-0834-9Published: 14 May 2014
Softcover ISBN: 978-1-4939-4646-4Published: 24 September 2016
eBook ISBN: 978-1-4939-0835-6Published: 13 May 2014
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: X, 319
Number of Illustrations: 20 b/w illustrations, 38 illustrations in colour
Topics: Human Genetics