Overview
- Editors:
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Josie Ursini-Siegel
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Lady Davis Institute for Medical Research, Montreal, Canada
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Nicole Beauchemin
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Goodman Cancer Research Centre, McGill University, Montreal, Canada
- Includes cutting-edge methods and protocols for tumor microenvironment research
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
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Table of contents (23 protocols)
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- Hidetoshi Mori, Robert D. Cardiff
Pages 1-12
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- Nicholas R. Bertos, Morag Park
Pages 13-25
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- Lian Willetts, David Bond, Konstantin Stoletov, John D. Lewis
Pages 27-37
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- Esterina D’Asti, Brian Meehan, Janusz Rak
Pages 39-58
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- José A. Jiménez-Torres, David J. Beebe, Kyung E. Sung
Pages 59-69
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- Eelco F. J. Meijer, James W. Baish, Timothy P. Padera, Dai Fukumura
Pages 71-85
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- Pardeep Heir, Michael Ohh
Pages 87-94
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- Yoon Kow Young, Alicia M. Bolt, Ryuhjin Ahn, Koren K. Mann
Pages 95-110
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- Kelly A. Pike, Caitlyn Hui, Connie M. Krawczyk
Pages 111-124
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- Camilla Salvagno, Karin E. de Visser
Pages 125-135
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- Joanne A. Hammill, Arya Afsahi, Jonathan L. Bramson, Christopher W. Helsen
Pages 137-157
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- Bertrand Allard, David Allard, John Stagg
Pages 159-177
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- Jitka Y. Sagiv, Sandra Voels, Zvi Granot
Pages 179-193
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- Khalid Al-Nedawi, Jolene Read
Pages 195-202
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- Victor Naumenko, Craig Jenne, Douglas J. Mahoney
Pages 217-230
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- Chirayu Chokshi, Manvir Dhillon, Nicole McFarlane, Chitra Venugopal, Sheila K. Singh
Pages 231-245
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About this book
This book covers core and emerging in vitro and in vivo protocols used to study how various components of the tumor microenvironment are established and subsequently interact with tumor cells to facilitate carcinogenesis. In addition, the book examines research topics including cellular and molecular biology approaches, in vivo genetic approaches, various “omics”-based strategies, therapeutic strategies to target the microenvironment, and, finally, advanced techniques in the fields of tissue engineering and nanotechnology. Written and validated in the laboratories of a number of trusted collaborating authors for the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, The Tumor Microenvironment: Methods and Protocols constitutes a compendium of techniques now available to a broad audience, including basic and clinician scientists, systems biologists, and biological engineers.
Editors and Affiliations
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Lady Davis Institute for Medical Research, Montreal, Canada
Josie Ursini-Siegel
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Goodman Cancer Research Centre, McGill University, Montreal, Canada
Nicole Beauchemin