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Multipotent Stem Cells of the Hair Follicle

Methods and Protocols

  • Book
  • © 2016

Overview

Editors:
  1. Robert M Hoffman

    1. University of California, AntiCancer, Inc., San Diego, USA

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1453)

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Table of contents (18 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Introduction to Hair-Follicle-Associated Pluripotent Stem Cells

    • Robert M. Hoffman
    Pages 1-5

  3. Nestin-Based Reporter Transgenic Mouse Lines

    • John Mignone, Natalia Peunova, Grigori Enikolopov
    Pages 7-14

  4. Discovery of HAP Stem Cells

    • Lingna Li, Robert M. Hoffman
    Pages 15-20

  5. Peripheral-Nerve and Spinal-Cord Regeneration in Mice Using Hair-Follicle-Associated Pluripotent (HAP) Stem Cells

    • Yasuyuki Amoh, Kensei Katsuoka, Robert M. Hoffman
    Pages 21-32

  6. Construction of Tissue-Engineered Nerve Conduits Seeded with Neurons Derived from Hair-Follicle Neural Crest Stem Cells

    • Fang Liu, Haiyan Lin, Chuansen Zhang
    Pages 33-38

  7. Nestin-Expressing Hair-Follicle-Associated Pluripotent (HAP) Stem Cells Promote Whisker Sensory-Nerve Growth in Long-Term 3D-Gelfoam® Histoculture

    • Sumiyuki Mii, Jennifer Duong, Yasunori Tome, Aisada Uchugonova, Fang Liu, Yasuyuki Amoh et al.
    Pages 39-47

  8. Isolation and Culture of Neural Crest Stem Cells from Human Hair Follicles

    • Ruifeng Yang, Xiaowei Xu
    Pages 49-55

  9. Isolation of Mouse Hair Follicle Bulge Stem Cells and Their Functional Analysis in a Reconstitution Assay

    • Ying Zheng, Jen-Chih Hsieh, Julia Escandon, George Cotsarelis
    Pages 57-69

  10. Hair Follicle Regeneration by Transplantation of a Bioengineered Hair Follicle Germ

    • Katsunari Tezuka, Koh-ei Toyoshima, Takashi Tsuji
    Pages 71-84

  11. Hair Induction by Cultured Mesenchymal Cells Using Sphere Formation

    • Kazuo Kishi, Ruka Hayashi
    Pages 85-92

  12. Stereological Quantification of Cell-Cycle Kinetics and Mobilization of Epithelial Stem Cells during Wound Healing

    • Eduardo Martínez-Martínez, Eileen Uribe-Querol, Claudio I. Galván-Hernández, Gabriel Gutiérrez-Ospina
    Pages 93-107

  13. Culture of Dermal Papilla Cells from Ovine Wool Follicles: An In Vitro Model for Papilla Size Determination

    • Nicholas W. Rufaut, Allan J. Nixon, Rodney D. Sinclair
    Pages 109-121

  14. Isolation and Fluorescence-Activated Cell Sorting of Mouse Keratinocytes Expressing β-Galactosidase

    • Maria Kasper, Rune ToftgÃ¥rd, Viljar Jaks
    Pages 123-136

  15. Protocols for Ectopic Hair Growth from Transplanted Whisker Follicles on the Spinal Cord of Mice

    • Wenluo Cao, Fang Liu, Yasuyuki Amoh, Robert M. Hoffman
    Pages 137-144

  16. Protocols for Gelfoam® Histoculture of Hair-Shaft-Producing Mouse Whisker Follicles Containing Nestin-GFP-Expressing Hair-Follicle-Associated Pluripotent (HAP) Stem Cells for Long Time Periods

    • Wenluo Cao, Fang Liu, Yasuyuki Amoh, Robert M. Hoffman
    Pages 145-150

  17. Protocols for Efficient Differentiation of Hair Follicle-Associated Pluripotent (HAP) Stem Cells to Beating Cardiac Muscle Cells

    • Masateru Yashiro, Sumiyuki Mii, Ryoichi Aki, Yuko Hamada, Nobuko Arakawa, Katsumasa Kawahara et al.
    Pages 151-159

  18. Highly Efficient Neural Differentiation of CD34-Positive Hair-Follicle-Associated Pluripotent Stem Cells Induced by Retinoic Acid and Serum-Free Medium

    • Mohsen Sagha, Nowruz Najafzadeh
    Pages 161-172

  19. Protocols for Cryopreservation of Intact Hair Follicle That Maintain Pluripotency of Nestin-Expressing Hair-Follicle-Associated Pluripotent (HAP) Stem Cells

    • Satoshi Kajiura, Sumiyuki Mii, Ryoichi Aki, Yuko Hamada, Nobuko Arakawa, Katsumasa Kawahara et al.
    Pages 173-178

  20. Back Matter

    Pages 179-180
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Keywords

About this book

This volume discusses methods for the study of multipotent and pluripotent stem cells of the hair follicle. The stem cells described are involved in both the growth of the hair follicle and its production of the hair shaft, as well as the growth of the hair follicle sensory nerve. Multipotent Stem Cells of the Hair Follicle: Methods and Protocols also explores very unexpected results such as that of the hair follicle-associated-pluripotent (HAP) stem cells, which not only have the capability for regenerating the hair follicle sensory nerve, but also can differentiate ex vivo and in vivo to multiple cell types not associated with the hair follicle—these include glial cells, motor neurons, and beating cardiac muscle cells. The potential for HAP stem cells for regenerative medicine is also discussed in detail. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Multipotent Stem Cells of the Hair Follicle: Methods and Protocols is a valuable resource for researchers interested in this field.

 

Editors and Affiliations

  • University of California, AntiCancer, Inc., San Diego, USA

    Robert M Hoffman

About the editor

Robert M. Hoffman, Ph.D. Professor of Surgery, University of California, San Diego President, AntiCancer, Inc. San Diego, CA, USA.

Bibliographic Information

  • Book Title: Multipotent Stem Cells of the Hair Follicle

  • Book Subtitle: Methods and Protocols

  • Editors: Robert M Hoffman

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-3786-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-3784-4Published: 19 July 2016

  • Softcover ISBN: 978-1-4939-8138-0Published: 30 May 2018

  • eBook ISBN: 978-1-4939-3786-8Published: 18 July 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 180

  • Number of Illustrations: 9 b/w illustrations, 34 illustrations in colour

  • Topics: Cell Biology

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