Neurotransmitter Transporters

1. Front Matter

   Pages i-xiii

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2. Classical Radioligand Uptake and Binding Methods in Transporter Research: An Emphasis on the Monoamine Neurotransmitter Transporters

   • Sonja Sucic, Heinz Bönisch

   Pages 1-21

3. Tracer Flux Measurements to Study Outward Transport by Monoamine Neurotransmitter Transporters

   • Thomas Steinkellner, Felix P. Mayer, Tina Hofmaier, Marion Holy, Therese Montgomery, Birgit Eisenrauch et al.

   Pages 23-40

4. Interrogating the Activity of Ligands at Monoamine Transporters in Rat Brain Synaptosomes

   • John S. Partilla, Michael H. Baumann, Ann M. Decker, Bruce E. Blough, Richard B. Rothman

   Pages 41-52

5. Using High-Speed Chronoamperometry to Measure Biogenic Amine Release and Uptake In Vivo

   • Lynette C. Daws, W. Anthony Owens, Glenn M. Toney

   Pages 53-81

6. Experimental Data Guided Docking of Small Molecules into Homology Models of Neurotransmitter Transporters

   • Andreas Jurik, Amir Seddik, Gerhard F. Ecker

   Pages 83-89

7. Immunohistochemical Methods for the Study of the Expression of Low-Affinity Monoamine Transporters in the Brain

   • Franck Louis, Thomas Couroussé, Sophie Gautron

   Pages 91-108

8. Analysis of Neurotransmitter Transporter Posttranslational Modifications: Phosphorylation and Palmitoylation

   • James D. Foster, Danielle E. Rastedt, Sathyavathi ChallaSivaKanaka, Roxanne A. Vaughan

   Pages 109-128

9. Ensemble and Single Quantum Dot Fluorescence Methods in Neurotransmitter Transporter Research

   • Oleg Kovtun, Sandra J. Rosenthal

   Pages 129-141

10. Approaches for Investigating Transporter Endocytic Trafficking in Cell Lines and Native Preparations

    • Haley E. Melikian, Sijia Wu, Luke R. Gabriel

    Pages 143-171

11. Organic Cation Transport Measurements Using Fluorescence Techniques

    • Giuliano Ciarimboli, Eberhard Schlatter

    Pages 173-187

12. Animal Models for Depression and the Mode of Action of Neurotransmitter Transporter-Blocking Antidepressants

    • Deeba Khan, Marianne Ronovsky, Thomas Steinkellner, Michael Freissmuth, Harald H. Sitte, Daniela D. Pollak

    Pages 189-202

13. In Vivo Imaging of Dopamine Metabolism and Dopamine Transporter Function in the Human Brain

    • Matthäus Willeit, Ana Popovic, Lucie Bartova, Ulrich Sauerzopf, Martin Bauer, Nicole Praschak-Rieder

    Pages 203-220

14. Back Matter

    Pages 221-229

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The volume aims at providing an overview of cutting-edge methods and protocols used in neuroscience and neurological research. Neurotransmitter Transporters: Investigative Methods will be of interest to scientists, graduate students, and advanced undergraduates who seek an overview of methods and protocols in this field of neuroscience. It will also be of interest to physicians who are carrying out imaging and postmortem studies of neurotransmitter transporters in the human brain. Written for the popular Neuromethods series, chapters include the kind of detail and key implementation advice that ensures successful results in the laboratory.

Authoritative and practical, Neurotransmitter Transporters: Investigative Methods aims to ensure successful results in the further study of this vital field.

• neuroscience
• neurological research
• neurotransmitter transporters
• human brain
• neuronal activity

• Biomedical Center Institute of Pharmacology and Toxicology, University of Bonn, Bonn, Germany

  Heinz Bönisch

• Center of Physiology and Pharmacology Institute of Pharmacology, Medical University of Vienna, Vienna, Austria

  Harald H. Sitte

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