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The Nuclear Receptor Superfamily

Methods and Protocols

  • Book
  • © 2016

Overview

Editors:
  1. Iain J. McEwan, PhD

    1. Ins. of Med. Sci. Uni. of Aberdeen, Sch. of Medicine Med. Sci. and Nutrition, Aberdeen, United Kingdom

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1443)

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Table of contents (14 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. Introduction

    1. Front Matter

      Pages 1-1
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    2. The Nuclear Receptor Superfamily at Thirty

      • Iain J. McEwan
      Pages 3-9

  3. Nuclear Receptor Agonists and Antagonists

    1. Front Matter

      Pages 11-11
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    2. Lipid Homeostasis and Ligands for Liver X Receptors: Identification and Characterization

      • Jean-Marc A. Lobaccaro, Claude Beaudoin, Bagora Bayala, Silvère Baron, Amalia Trousson
      Pages 13-19

    3. Use of Differential Scanning Fluorimetry to Identify Nuclear Receptor Ligands

      • Kara A. DeSantis, Jeffrey L. Reinking
      Pages 21-30

    4. Drug-Discovery Pipeline for Novel Inhibitors of the Androgen Receptor

      • Kush Dalal, Ravi Munuganti, Hélène Morin, Nada Lallous, Paul S. Rennie, Artem Cherkasov
      Pages 31-54

  4. Nuclear Receptor Signalling

    1. Front Matter

      Pages 55-55
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    2. Use of BRET to Study Protein–Protein Interactions In Vitro and In Vivo

      • Shalini Dimri, Soumya Basu, Abhijit De
      Pages 57-78

    3. Studying Nuclear Receptor Complexes in the Cellular Environment

      • Fred Schaufele
      Pages 79-104

    4. Posttranslational Modifications of Steroid Receptors: Phosphorylation

      • Dagmara McGuinness, Iain J. McEwan
      Pages 105-117

    5. Mapping Protein–DNA Interactions Using ChIP-exo and Illumina-Based Sequencing

      • Stefan J. Barfeld, Ian G. Mills
      Pages 119-137

    6. Methods to Identify Chromatin-Bound Protein Complexes: From Genome-Wide to Locus-Specific Approaches

      • Charles E. Massie
      Pages 139-150

    7. Measuring the Expression of microRNAs Regulated by Androgens

      • Mauro Scaravilli, Kati Kivinummi, Tapio Visakorpi, Leena Latonen
      Pages 151-163

    8. Methods for Identifying and Quantifying mRNA Expression of Androgen Receptor Splicing Variants in Prostate Cancer

      • Yingming Li, Scott M. Dehm
      Pages 165-177

  5. Model Systems

    1. Front Matter

      Pages 179-179
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    2. Harvesting Human Prostate Tissue Material and Culturing Primary Prostate Epithelial Cells

      • Fiona M. Frame, Davide Pellacani, Anne T. Collins, Norman J. Maitland
      Pages 181-201

    3. In Vivo Imaging of Nuclear Receptor Transcriptional Activity

      • D. Alwyn Dart, Charlotte L. Bevan
      Pages 203-217

    4. Development and Characterization of Cell-Specific Androgen Receptor Knockout Mice

      • Laura O’Hara, Lee B. Smith
      Pages 219-248

  6. Back Matter

    Pages 249-250
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Keywords

About this book

This volume aims to describe a complementary range of molecular, cell biological, and in vivo protocols used to investigate the structure–function of nuclear receptors, together with experimental approaches that may lead to new drugs to selectively target nuclear receptor-associated diseases. The Nuclear Receptor Superfamily, Second Edition will benefit those starting out in the nuclear receptor research field as well as to more established researchers who wish to apply different methods to a particular receptor or research problem. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, The Nuclear Receptor Superfamily, Second Edition aims to ensure successful results in the further study of this vital field.

Editors and Affiliations

  • Ins. of Med. Sci. Uni. of Aberdeen, Sch. of Medicine Med. Sci. and Nutrition, Aberdeen, United Kingdom

    Iain J. McEwan, PhD

Bibliographic Information

  • Book Title: The Nuclear Receptor Superfamily

  • Book Subtitle: Methods and Protocols

  • Editors: Iain J. McEwan, PhD

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-3724-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2016

  • Hardcover ISBN: 978-1-4939-3722-6Published: 01 June 2016

  • Softcover ISBN: 978-1-4939-8120-5Published: 26 May 2018

  • eBook ISBN: 978-1-4939-3724-0Published: 31 May 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: X, 250

  • Number of Illustrations: 12 b/w illustrations, 32 illustrations in colour

  • Topics: Cell Biology

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