Non-Viral Gene Delivery Vectors

1. Front Matter

   Pages i-xiv

   PDF

2. Conventional Bolus Gene Delivery Vectors

   1. Front Matter

      Pages 1-1

      PDF

   2. Physical Chemical and Biomolecular Methods for the Optimization of Cationic Lipid-Based Lipoplexes In Vitro for the Gene Therapy Applications

      • Santosh K. Misra, Santanu Bhattacharya

      Pages 3-17

   3. Cationic Lipid-Based Nucleic Acid Vectors

      • Emile Jubeli, William P. D. Goldring, Michael D. Pungente

      Pages 19-32

   4. Lipoplexes from Non-viral Cationic Vectors: DOTAP-DOPE Liposomes and Gemini Micelles

      • Sara Falsini, Sandra Ristori

      Pages 33-43

   5. Anionic/Zwitterionic Lipid-Based Gene Vectors of pDNA

      • Ana L. Barrán-Berdón, Emilio Aicart, Elena Junquera

      Pages 45-61

   6. Elaboration and Physicochemical Characterization of Niosome-Based Nioplexes for Gene Delivery Purposes

      • Edilberto Ojeda, Mireia Agirre, Ilia Villate-Beitia, Mohamed Mashal, Gustavo Puras, Jon Zarate et al.

      Pages 63-75

   7. Quantitative Intracellular Localization of Cationic Lipid–Nucleic Acid Nanoparticles with Fluorescence Microscopy

      • Ramsey N. Majzoub, Kai K. Ewert, Cyrus R. Safinya

      Pages 77-108

   8. Targeted Delivery of Peptide-Tagged DNA Lipoplexes to Hepatocellular Carcinoma Cells

      • Mario Ariatti

      Pages 109-136

   9. Lipoplexes Strengthened by Anionic Polymers: Easy Preparation of Highly Effective siRNA Vectors Based on Cationic Lipids and Anionic Polymers

      • Danielle Campiol Arruda, Anne Schlegel, Pascal Bigey, Virginie Escriou

      Pages 137-148

   10. Polymer Based Gene Silencing: In Vitro Delivery of SiRNA

       • Margarida I. Simão Carlos, Andreas Schätzlein, Ijeoma Uchegbu

       Pages 149-157

   11. Polyallylamine Derivatives: Novel NonToxic Transfection Agents

       • Magdalena Wytrwal, Chantal Pichon

       Pages 159-174

   12. Biodegradable Three-Layered Micelles and Injectable Hydrogels

       • Daniel G. Abebe, Rima Kandil, Teresa Kraus, Maha Elsayed, Tomoko Fujiwara, Olivia M. Merkel

       Pages 175-185

   13. Cationic Lipid-Coated Polyplexes (Lipopolyplexes) for DNA and Small RNA Delivery

       • Alexander Ewe, Achim Aigner

       Pages 187-200

   14. Preparation of Targeted Anionic Lipid-Coated Polyplexes for MicroRNA Delivery

       • Xiaomeng Huang, Mengzi Zhang, Xinmei Wang, L. James Lee, Robert J. Lee

       Pages 201-213

3. Stimuli-Responsive Bolus Gene Delivery Vectors

   1. Front Matter

      Pages 215-215

      PDF

   2. Characterization and Investigation of Redox-Sensitive Liposomes for Gene Delivery

      • Daniele Pezzoli, Elena Tallarita, Elena Rosini, Gabriele Candiani

      Pages 217-233

   3. From Artificial Amino Acids to Sequence-Defined Targeted Oligoaminoamides

      • Stephan Morys, Ernst Wagner, Ulrich Lächelt

      Pages 235-258

   4. Gene Delivery Method Using Photo-Responsive Poly(β-Amino Ester) as Vectors

      • Nan Zheng, Yang Liu, Jianjun Cheng

      Pages 259-267

   5. Thermo-Responsive Polyplex Micelles with PEG Shells and PNIPAM Layer to Protect DNA Cores for Systemic Gene Therapy

      • Junjie Li, Zengshi Zha, Zhishen Ge

      Pages 269-276

This volume provides readers with a wide collection of the latest and readily reproducible technical protocols available in the field of non-viral gene delivery vectors. The chapters in this book are organized into three major parts: Part I is a section on conventional bolus gene delivery vectors that introduces typical transfection approaches relying on the addition of transfectants to the cell culture medium where the cells are grown in; Part II covers stimuli-responsive bolus transfectants and topics on gene delivery complexes made of smart polymers or stimuli-responsive polymers that change according to the environment they are in and delivered by dripping into cells; Part III discusses examples of substrate-mediated gene delivery—also termed reverse transfection—and the immobilization of a gene delivery vector onto a surface as opposed to more typical bolus delivery from the medium. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and practical, Non-Viral Gene Delivery Vectors: Methods and Protocols is written for experimentalists, and is an essential part of many laboratory bookshelves. This book will help novice and professionals alike succeed in their research in this field.

• conventional bolus gene delivery vectors
• cell culture medium
• stimuli-responsive bolus transfectants
• polymers
• reverse transfection
• solid-phase delivery
• cationic lipid-based lipoplexes
• zwitterionic lipid-based lipoplexes
• polymer-strenthened
• lipid-coated

• Polytechnic University of Milan, Milan, Italy

  Gabriele Candiani

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