Skip to main content
  • Book
  • © 2016

Angiogenesis Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1430)

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 139.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

This is a preview of subscription content, log in via an institution to check for access.

Table of contents (27 protocols)

  1. Front Matter

    Pages i-xi
  2. Microscopic Assessment

    1. Front Matter

      Pages 1-1
    2. Assessing Tumor Angiogenesis in Histological Samples

      • Jia-Min Pang, Nicholas Jene, Stephen B. Fox
      Pages 3-33
    3. Immunohistochemical Methods for Measuring Tissue Lymphangiogenesis

      • Daniel J. Royston, Steven Clasper, David G. Jackson
      Pages 35-48
    4. Immunohistochemical Assessment of Leukocyte Involvement in Angiogenesis

      • Narmeen S. Ahmad, Stewart G. Martin, Sarah J. Storr
      Pages 49-57
  3. Cell Isolation Techniques

    1. Front Matter

      Pages 59-59
    2. Isolation, Culture, and Characterization of Vascular Smooth Muscle Cells

      • Jessal J. Patel, Salil Srivastava, Richard C. M. Siow
      Pages 91-105
    3. Isolation and Transfection of Primary Culture Bovine Retinal Pericytes

      • Vincent A. Primo, Joseph F. Arboleda-Velasquez
      Pages 107-117
  4. In Vitro Techniques

    1. Front Matter

      Pages 119-119
    2. Tube-Forming Assays

      • Ryan M. Brown, Christopher J. Meah, Victoria L. Heath, Iain B. Styles, Roy Bicknell
      Pages 149-157
    3. In Vitro Coculture Assays of Angiogenesis

      • Mark Richards, Harry Mellor
      Pages 159-166
    4. Spheroid-Based In Vitro Angiogenesis Model

      • Larissa Pfisterer, Thomas Korff
      Pages 167-177
    5. Human Arterial Ring Angiogenesis Assay

      • Giorgio Seano, Luca Primo
      Pages 191-203
    6. A Modified Aortic Ring Assay to Assess Angiogenic Potential In Vitro

      • Nina Zippel, Yindi Ding, Ingrid Fleming
      Pages 205-219
    7. Static and Dynamic Assays of Cell Adhesion Relevant to the Vasculature

      • Lynn M. Butler, Helen M. McGettrick, Gerard B. Nash
      Pages 231-248

About this book

In addition to updating important core techniques this third edition presents new chapters on assessing leukocyte involvement in angiogenesis, lymphatic cell and pericyte isolation techniques, spheroid and arterial ring based in vitro assays, and on pericyte involvement in angiogenesis. Angiogenesis Protocols, Third Edition also guides readers through new protocols on chorioallantoic membrane models, corneal pocket assays to assess angio- and lymphangiogenesis, models of muscle angiogenesis, and use of zebrafish embryos to study vascular angiogenesis and senescence. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Angiogenesis Protocols, Third Edition provides not only a practical handbook for key techniques, but also an informative and enjoyable read for all those interested, no matter how directly, in angiogenesis.

Editors and Affiliations

  • Nottingham, United Kingdom

    Stewart G. Martin

  • UNIV BIRMINGHAM/MEDICAL SCHOOL, BIRMINGHAM B15 2TT, United Kingdom

    Peter W. Hewett

Bibliographic Information

  • Book Title: Angiogenesis Protocols

  • Editors: Stewart G. Martin, Peter W. Hewett

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-3628-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-3626-7Published: 13 May 2016

  • Softcover ISBN: 978-1-4939-8099-4Published: 26 May 2018

  • eBook ISBN: 978-1-4939-3628-1Published: 12 May 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 3

  • Number of Pages: XI, 404

  • Number of Illustrations: 20 b/w illustrations, 42 illustrations in colour

  • Topics: Cell Biology

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 139.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access