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The Mitotic Spindle

Methods and Protocols

  • Book
  • © 2016

Overview

Editors:
  1. Paul Chang

    1. Massachusetts Institute of Technolo, Cambridge, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Ryoma Ohi

    1. Dept of Cell & Developmental Biolog, Vanderbilt University, Nashville, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1413)

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Table of contents (25 protocols)

  1. Front Matter

    Pages i-xiii
    Download chapter PDF

  2. Methods Focused on Cytology

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Using Fluorescence Microscopy to Study Mitosis

      • Sai K. Balchand, Barbara J. Mann, Patricia Wadsworth
      Pages 3-14

    3. Using Photoactivatable GFP to Study Microtubule Dynamics and Chromosome Segregation

      • Bin He, Daniela Cimini
      Pages 15-31

  3. Methods Focused on Microtubules and the Mitotic Spindle

    1. Front Matter

      Pages 33-33
      Download chapter PDF

    2. Purification and Fluorescent Labeling of Tubulin from Xenopus laevis Egg Extracts

      • Aaron C. Groen, Timothy J. Mitchison
      Pages 35-45

    3. Measuring the Effects of Microtubule-Associated Proteins on Microtubule Dynamics In Vitro

      • Marija Zanic
      Pages 47-61

    4. Imaging and Quantifying the Dynamics of γ-Tubulin at Microtubule Minus Ends in Mitotic Spindles

      • Nicolas Lecland, Jens Lüders
      Pages 63-75

    5. Visualizing and Analyzing Branching Microtubule Nucleation Using Meiotic Xenopus Egg Extracts and TIRF Microscopy

      • Matthew King, Sabine Petry
      Pages 77-85

    6. Encapsulation of Xenopus Egg and Embryo Extract Spindle Assembly Reactions in Synthetic Cell-Like Compartments with Tunable Size

      • Matthew C. Good
      Pages 87-108

  4. Methods Focused on Kinetochores and the Kinetochore-Microtubule Interface

    1. Front Matter

      Pages 109-109
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    2. In Vitro Kinetochore Assembly

      • Matthew D. D. Miell, Aaron F. Straight
      Pages 111-133

    3. Biochemical and Structural Analysis of Kinetochore Histone-Fold Complexes

      • Tatsuya Nishino, Tatsuo Fukagawa
      Pages 135-146

    4. Measuring Kinetochore–Microtubule Attachment Stability in Cultured Cells

      • Keith F. DeLuca, Jacob A. Herman, Jennifer G. DeLuca
      Pages 147-168

    5. Studying Kinetochores In Vivo Using FLIM-FRET

      • Tae Yeon Yoo, Daniel J. Needleman
      Pages 169-186

  5. Methods Focused on the Spindle Pole

    1. Front Matter

      Pages 187-187
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    2. Purification of Fluorescently Labeled Saccharomyces cerevisiae Spindle Pole Bodies

      • Kimberly K. Fong, Beth Graczyk, Trisha N. Davis
      Pages 189-195

    3. A Cell-Free System for Real-Time Analyses of Centriole Disengagement and Centriole-to-Centrosome Conversion

      • Rajesh Kumar Soni, Meng-Fu Bryan Tsou
      Pages 197-206

    4. Assays to Study Mitotic Centrosome and Spindle Pole Assembly and Regulation

      • Vladimir Joukov, Johannes C. Walter, Arcangela De Nicolo
      Pages 207-235

  6. Methods Focused on the Cellular Functions of Microtubule Motor Proteins

    1. Front Matter

      Pages 237-237
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Keywords

About this book

This volume includes a series of protocols focused on mitotic spindle assembly and function. The methods covered in this book feature a broad range of techniques from basic microscopy to the study of spindle physiologies relevant to cancer. These methods can be applied to diverse model systems that range from the cell-free Xenopus egg extract system to the moss Physcomitrella patens, in an effort to demonstrate the key contributions made by researchers using multiple model organisms. Chapters in The Mitotic Spindle: Methods and Protocols integrate cutting-edge technologies that have only become available due to the cross-disciplinary efforts, such as ATP analogue sensitive inhibition of mitotic kinases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips ontroubleshooting and avoiding known pitfalls.


Thorough and informative, The Mitotic Spindle: Methods and Protocols, is a valuable resource for researchers who are new to mitosis or are already experts in the field.

 

Editors and Affiliations

  • Massachusetts Institute of Technolo, Cambridge, USA

    Paul Chang

  • Dept of Cell & Developmental Biolog, Vanderbilt University, Nashville, USA

    Ryoma Ohi

Bibliographic Information

  • Book Title: The Mitotic Spindle

  • Book Subtitle: Methods and Protocols

  • Editors: Paul Chang, Ryoma Ohi

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-3542-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-3540-6Published: 20 May 2016

  • Softcover ISBN: 978-1-4939-8072-7Published: 26 May 2018

  • eBook ISBN: 978-1-4939-3542-0Published: 19 May 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 424

  • Number of Illustrations: 27 b/w illustrations, 52 illustrations in colour

  • Topics: Membrane Biology, Biological Microscopy

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