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  • © 2016

Transposons and Retrotransposons

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1400)

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Table of contents (26 protocols)

  1. Front Matter

    Pages i-xii
  2. Study of Transposable Elements and Their Genomic Impact

    • Martin Muñoz-Lopez, Raquel Vilar-Astasio, Pablo Tristan-Ramos, Cesar Lopez-Ruiz, Jose L. Garcia-Pérez
    Pages 1-19
  3. Bacterial Group II Introns: Identification and Mobility Assay

    • Nicolás Toro, María Dolores Molina-Sánchez, Rafael Nisa-Martínez, Francisco Martínez-Abarca, Fernando Manuel García-Rodríguez
    Pages 21-32
  4. In Silico Methods to Identify Exapted Transposable Element Families

    • LeeAnn Ramsay, Guillaume Bourque
    Pages 33-45
  5. RNA-Seq Analysis to Measure the Expression of SINE Retroelements

    • Ángel Carlos Román, Antonio Morales-Hernández, Pedro M. Fernández-Salguero
    Pages 107-116
  6. LINE Retrotransposition Assays in Saccharomyces cerevisiae

    • Axel V. Horn, Jeffrey S. Han
    Pages 131-137
  7. LINE-1 Cultured Cell Retrotransposition Assay

    • Huira C. Kopera, Peter A. Larson, John B. Moldovan, Sandra R. Richardson, Ying Liu, John V. Moran
    Pages 139-156
  8. L1 Retrotransposition in Neural Progenitor Cells

    • Alysson R. Muotri
    Pages 157-163
  9. Characterization of Engineered L1 Retrotransposition Events: The Recovery Method

    • David Cano, Santiago Morell, Andres J. Pulgarin, Suyapa Amador, Jose L. Garcia-Pérez
    Pages 165-182
  10. The Engineered SVA Trans-mobilization Assay

    • Anja Bock, Gerald G. Schumann
    Pages 203-222
  11. Detection of LINE-1 RNAs by Northern Blot

    • Prescott Deininger, Victoria P. Belancio
    Pages 223-236
  12. Immunodetection of Human LINE-1 Expression in Cultured Cells and Human Tissues

    • Reema Sharma, Nemanja Rodić, Kathleen H. Burns, Martin S. Taylor
    Pages 261-280
  13. Cellular Localization of Engineered Human LINE-1 RNA and Proteins

    • Aurélien J. Doucet, Eugénia Basyuk, Nicolas Gilbert
    Pages 281-297
  14. Purification of L1-Ribonucleoprotein Particles (L1-RNPs) from Cultured Human Cells

    • Prabhat K. Mandal, Haig H. Kazazian Jr.
    Pages 299-310

About this book

This volume covers the latest protocols designed to identify and characterize TEs in genomes, ancient or recently inserted. Additionally, this book includes a series of protocols designed to understand how host genomes act to regulate the activity of TEs, from elegant genetic mobilization assays to key biochemical methods. Finally, this book also includes chapters that describe how TEs can be used for biotechnological applications. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Transposons and Retrotransposons: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Editors and Affiliations

  • Institute of Genetics and Molecular Medi, Genyo (Center for Genomics and Oncologic, Granada, Spain

    Jose L. Garcia-Pérez

Bibliographic Information

  • Book Title: Transposons and Retrotransposons

  • Book Subtitle: Methods and Protocols

  • Editors: Jose L. Garcia-Pérez

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-3372-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-3370-9Published: 20 February 2016

  • Softcover ISBN: 978-1-4939-8033-8Published: 31 March 2018

  • eBook ISBN: 978-1-4939-3372-3Published: 19 February 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 434

  • Number of Illustrations: 17 b/w illustrations, 56 illustrations in colour

  • Topics: Human Genetics

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access