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Receptor and Ion Channel Detection in the Brain

Methods and Protocols

  • Book
  • © 2016

Overview

Editors:
  1. Rafael Luján

    1. Research Institute on Neurological Disabilities (IDINE), Department of Medical Sciences, School of Medicine, University of Castilla- La Mancha, Albacete, Spain

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    You can also search for this editor in PubMed   Google Scholar

  2. Francisco Ciruela

    1. Pharmacology Unit, Department of Pathology and Experimental Therapeutics, School of Medicine, IDIBELL, University of Barcelona, Barcelona, Spain

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and key protocols to study receptors and ion channels
  • Provides step-by-step detail essential for reproducible results
  • Contains well-organized themes, key notes and expert implementation advice
  • Includes methods applicable to the basic research and clinical laboratories
  • Suitable for both novice and experienced investigators

Part of the book series: Neuromethods (NM, volume 110)

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Table of contents (29 protocols)

  1. Front Matter

    Pages i-xviii
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  2. Molecular Techniques

    1. Front Matter

      Pages 1-1
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    2. Production of High-Quality Antibodies for the Study of Receptors and Ion Channels

      • Masahiko Watanabe
      Pages 3-18

    3. Co-immunoprecipitation from Brain

      • Xavier Morató, Dasiel O. Borroto-Escuela, Kjell Fuxe, Víctor Fernández-Dueñas, Francisco Ciruela
      Pages 19-29

    4. Subsynaptic Membrane Fractionation

      • Paula M. Canas, Rodrigo A. Cunha
      Pages 31-37

    5. Investigation of Neurotransmitter Receptors in Brain Slices Using Cell Surface Biotinylation

      • Elek Molnár
      Pages 39-48

    6. Single Nanoparticle Tracking of Surface Ion Channels and Receptors in Brain Cells

      • Juan Varela, Julien Dupuis, Laurent Groc
      Pages 49-58

    7. Radioligand Binding Detection of Receptors in Brain Membranes

      • Fuencisla Pilar-Cuéllar, Alvaro Díaz, Emilio Garro-Martínez, Alicia Martín, Beatriz Romero, Elsa M. Valdizán
      Pages 59-76

    8. Recombinant Alphavirus-Mediated Expression of Ion Channels and Receptors in the Brain

      • Markus U. Ehrengruber, Kenneth Lundstrom
      Pages 77-97

    9. Fluorescent Ligands and TR-FRET to Study Receptor–Receptor Interactions in the Brain

      • Víctor Fernández-Dueñas, Thierry Durroux, Francisco Ciruela
      Pages 99-107

    10. In Situ Proximity Ligation Assay to Study and Understand the Distribution and Balance of GPCR Homo- and Heteroreceptor Complexes in the Brain

      • Dasiel O. Borroto-Escuela, Beth Hagman, Miles Woolfenden, Luca Pinton, Antonio Jiménez-Beristain, Julia Oflijan et al.
      Pages 109-124

  3. Neuroanatomical Techniques

    1. Front Matter

      Pages 125-125
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    2. Fluorescent In Situ Hybridization for Sensitive and Specific Labeling

      • Miwako Yamasaki, Masahiko Watanabe
      Pages 127-142

    3. Autoradiographic Visualization of G Protein-Coupled Receptors in Brain

      • Rebeca Vidal, Raquel Linge, María Josefa Castillo, Josep Amigó, Elsa M. Valdizán, Elena Castro
      Pages 143-156

    4. Analysis of the Expression Profile and Regional Distribution of Neurotransmitter Receptors and Ion Channels in the Central Nervous System Using Histoblots

      • Elek Molnár
      Pages 157-170

    5. Immunohistochemistry for Ion Channels and Their Interacting Molecules: Tips for Improving Antibody Accessibility

      • Kohtarou Konno, Masahiko Watanabe
      Pages 171-178

    6. Localization of GFP-Tagged Proteins at the Electron Microscope

      • Sara Gil-Perotin, A. Cebrián-Silla, V. Herranz-Pérez, P. García-Belda, S. Gil-García, M. Fil et al.
      Pages 179-190

    7. Pre-embedding Methods for the Localization of Receptors and Ion Channels

      • Rafael Luján
      Pages 191-210

    8. Post-embedding Immunohistochemistry in the Localisation of Receptors and Ion Channels

      • Rafael Luján, Masahiko Watanabe
      Pages 211-232

    9. High-Resolution Localization of Membrane Proteins by SDS-Digested Freeze-Fracture Replica Labeling (SDS-FRL)

      • Harumi Harada, Ryuichi Shigemoto
      Pages 233-245
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Keywords

About this book

Receptor and Ion Channel Detection in the Brain provides state-of-the-art and up-to-date methodological information on molecular, neuroanatomical and functional techniques that are currently used to study neurotransmitter receptors and ion channels in the brain. The chapters have been contributed by world-wide recognized neuroscientists who explain in an easy and detailed way well established and tested protocols embracing molecular, cellular, subcellular, anatomical and electrophysiological aspects of the brain. This comprehensive and practical manual is presented in a simple, step-by-step manner for laboratory use, and also offers unambiguous detail and key implementation advice that proves essential for successful results and facilitate choosing the best method for the target proteins under study. This work serves as a useful guide for young researchers and students in training as well as for neurologists and established scientists who wish to extend their repertoire of techniques.

Editors and Affiliations

  • Research Institute on Neurological Disabilities (IDINE), Department of Medical Sciences, School of Medicine, University of Castilla- La Mancha, Albacete, Spain

    Rafael Luján

  • Pharmacology Unit, Department of Pathology and Experimental Therapeutics, School of Medicine, IDIBELL, University of Barcelona, Barcelona, Spain

    Francisco Ciruela

Bibliographic Information

  • Book Title: Receptor and Ion Channel Detection in the Brain

  • Book Subtitle: Methods and Protocols

  • Editors: Rafael Luján, Francisco Ciruela

  • Series Title: Neuromethods

  • DOI: https://doi.org/10.1007/978-1-4939-3064-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2016

  • Hardcover ISBN: 978-1-4939-3063-0Published: 02 February 2016

  • Softcover ISBN: 978-1-4939-7983-7Published: 31 March 2018

  • eBook ISBN: 978-1-4939-3064-7Published: 02 February 2016

  • Series ISSN: 0893-2336

  • Series E-ISSN: 1940-6045

  • Edition Number: 1

  • Number of Pages: XVIII, 475

  • Topics: Neurosciences, Neurobiology, Biological Techniques

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