Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 1359)
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Table of contents (32 protocols)
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Front Matter
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Reviews on General Topics
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Front Matter
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Protocols of Somatic Embryogenesis in Selected Important Horticultural Plants
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Front Matter
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About this book
This volume presents an overview of recent advances, innovative applications, and future prospects of in vitro embryogenesis in higher plants. The book's chapters are divided into five parts: Part I contains reviews on general topics (microspore; zygotic and somatic embryogenesis; in vitro and in vivo asexual embryogenesis; advances on the genetic, physiological, and proteomic knowledge of somatic embryo formation; role of apoptosis and mitochondria in somatic embryogenesis; and innovation in the use of bioreactors). The remaining four parts discuss step-wise protocols on somatic embryogenesis in selected horticultural plants (Part II); forest trees (Part III); gametic embryogenesis (Part IV); and pivotal topics, such as the detection of epigenetics modifications during microspore embryogenesis, the in vitro embryogenesis and plant regenerations from isolated zygotes, the synthetics seed production, the induction and maturation of somatic embryos, and the cryostorage of embryogenic cultures (Part V). Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and thorough, In Vitro Embryogenesis in Higher Plants, is a useful source of information and ideas for plant tissue culturists, cell biologists, embryologists, horticulturists, and operators of commercial nurseries. This book will introduce the fascinating work in in vitro embryogenesis in higher plants to students and young scientists.
Editors and Affiliations
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Dipartimento DEMETRA, Università degli Studi di Palermo, Palermo, Italy
Maria Antonietta Germana
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National Research Council (CNR) IVALSA/Istituto per la Valorizzazione, Sesto Fiorentino, Florence, Italy
Maurizio Lambardi
About the editors
Maria Antonietta Germana' Dipartimento DEMETRA Università degli Studi di Palermo Viale Delle Scienze, 11 90128 Palermo -Italy e-mail: mariaantonietta.germana@unipa.it Tel. +39-09123896094 Fax: +39-09123860813 Cell. +39 388 0400044 Cell. +39 320 8397040
Maurizio Lambardi, CNR-IVALSA/Istituto per la Valorizzazione del Legno e delle Specie Arboree, Polo Scientifico, via Madonna del Piano n. 10, 50019 Sesto Fiorentino (Firenze), Italy
Email: lambardi@ivalsa.cnr.it Tel: +39-055-5225685
Bibliographic Information
Book Title: In Vitro Embryogenesis in Higher Plants
Editors: Maria Antonietta Germana, Maurizio Lambardi
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-3061-6
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2016
Hardcover ISBN: 978-1-4939-3060-9Published: 02 December 2015
Softcover ISBN: 978-1-4939-7982-0Published: 29 March 2019
eBook ISBN: 978-1-4939-3061-6Published: 30 November 2015
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XVIII, 558
Number of Illustrations: 3 b/w illustrations, 66 illustrations in colour
Topics: Plant Sciences