Cell Cycle Oscillators

1. Front Matter

   Pages i-xii

   PDF

2. Reviews

   1. Front Matter

      Pages 1-1

      PDF

   2. Cell Cycle Control: A System of Interlinking Oscillators

      • Randy Y. C. Poon

      Pages 3-19

   3. Model Organisms for Studying the Cell Cycle

      • Zhaohua Tang

      Pages 21-57

   4. Role of Computational Modeling in Understanding Cell Cycle Oscillators

      • Attila Csikász-Nagy, Ivan Mura

      Pages 59-70

   5. E2F Transcription Factors Control the Roller Coaster Ride of Cell Cycle Gene Expression

      • Ingrid Thurlings, Alain de Bruin

      Pages 71-88

3. Methods

   1. Front Matter

      Pages 89-89

      PDF

   2. Cell Synchronization of Mouse Embryonic Fibroblasts

      • Michael J. Thwaites, Courtney H. Coschi, Christian E. Isaac, Frederick A. Dick

      Pages 91-99

   3. Cell Cycle Synchronization in Xenopus Egg Extracts

      • Peter J. Gillespie, Julia Neusiedler, Kevin Creavin, Gaganmeet Singh Chadha, J. Julian Blow

      Pages 101-147

   4. Elutriation for Cell Cycle Synchronization in Fission Yeast

      • Kazunori Kume

      Pages 149-155

   5. Spatiotemporal Investigation of Phosphorylation Events During Cell Cycle Progression

      • Lilia Gheghiani, Olivier Gavet

      Pages 157-171

   6. Cell Cycle Dynamics of Proteins and Post-translational Modifications Using Quantitative Immunofluorescence

      • Karen Akopyan, Arne Lindqvist, Erik Müllers

      Pages 173-183

   7. Building a Synthetic Transcriptional Oscillator

      • Matthaeus Schwarz-Schilling, Jongmin Kim, Christian Cuba, Maximilian Weitz, Elisa Franco, Friedrich C. Simmel

      Pages 185-199

   8. The Use of SNAP Labeling to Study Cell Cycle Oscillatory Proteins

      • Christine Greil, Marie Follo, Monika Engelhardt, Ralph Wäsch

      Pages 201-208

   9. A Computational Method for Identifying Yeast Cell Cycle Transcription Factors

      • Wei-Sheng Wu

      Pages 209-219

   10. Measuring Activity and Specificity of Protein Phosphatases

       • Brendan L. Powers, Michael Melesse, Christie L. Eissler, Harry Charbonneau, Mark C. Hall

       Pages 221-235

   11. Combining the Optimized Yeast Cytosine Deaminase Protein Fragment Complementation Assay and an In Vitro Cdk1 Targeting Assay to Study the Regulation of the γ-Tubulin Complex

       • Po Hien Ear, Jacqueline Kowarzyk, Michael J. Booth, Diala Abd-Rabbo, Kristian Shulist, Conrad Hall et al.

       Pages 237-257

   12. Cell Cycle Synchronization Using a Microfluidic Synchronizer for Fission Yeast Cells

       • Shujing Wang, Chunxiong Luo

       Pages 259-268

   13. Detection of Protein–Protein Interactions in Tobacco BY-2 Cells Using Bimolecular Fluorescence Complementation

       • Gemma S. Puts, Natasha Spadafora

       Pages 269-277

   14. Tracking the Cyclin B1-GFP Sensor to Profile the Pattern of Mitosis Versus Mitotic Bypass

       • Victoria Griesdoorn, M. Rowan Brown, Marie Wiltshire, Paul J. Smith, Rachel J. Errington

       Pages 279-285

​ This volume brings together a unique collection of protocols that cover standard, novel, and specialized techniques. Cell Cycle Oscillators: Methods and Protocols guides readers through recent progress in the field from both holistic and reductionist perspectives, providing the latest developments in molecular biology techniques, biochemistry, and computational analysis used for studying oscillatory networks. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Cell Cycle Oscillators: Methods and Protocols will serve as an invaluable reference to gain further insight into the complex and incompletely understood processes that are involved in the cell cycle and its regulation by oscillatory networks.

• cell cycle
• cell division cycle
• living systems
• oscillatory networks
• proliferative diseases
• reproduction
• small molecules
• Cell Cycle Analysis

• Department of Oncology, University of Oxford, Oxford, United Kingdom

  Amanda S. Coutts

• Scientific writer, London, United Kingdom

  Louise Weston

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