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Methods in Mouse Atherosclerosis

  • Book
  • © 2015

Overview

Editors:
  1. Vicente Andrés

    1. Laboratory of Molecular and Genetic Cardiovascular Pathophysiology, Centro Nacional de Investigaciones Cardiovascular Carlos III (CNIC), Madrid, Spain

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  2. Beatriz Dorado

    1. Laboratory of Molecular and Genetic Cardiovascular Pathophysiology, Centro Nacional de Investigaciones Cardiovascular Carlos III (CNIC), Madrid, Spain

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1339)

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Table of contents (29 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Use of Mouse Models in Atherosclerosis Research

    • Godfrey S. Getz, Catherine A. Reardon
    Pages 1-16

  3. Cytokines and Immune Responses in Murine Atherosclerosis

    • Pascal J. H. Kusters, Esther Lutgens
    Pages 17-40

  4. Intravital Microscopy for Atherosclerosis Research

    • Remco T. A. Megens, Oliver Soehnlein
    Pages 41-60

  5. Noninvasive Molecular Imaging of Mouse Atherosclerosis

    • Martina A. McAteer, Robin P. Choudhury
    Pages 61-83

  6. Oil Red O and Hematoxylin and Eosin Staining for Quantification of Atherosclerosis Burden in Mouse Aorta and Aortic Root

    • M. Jesús Andrés-Manzano, Vicente Andrés, Beatriz Dorado
    Pages 85-99

  7. Isolation, Culture, and Polarization of Murine Bone Marrow-Derived and Peritoneal Macrophages

    • Inés Pineda-Torra, Matthew Gage, Alba de Juan, Oscar M. Pello
    Pages 101-109

  8. Isolation of Mouse Primary Aortic Endothelial Cells by Selection with Specific Antibodies

    • Pedro Molina-Sánchez, Vicente Andrés
    Pages 111-117

  9. Isolation and Culture of Aortic Smooth Muscle Cells and In Vitro Calcification Assay

    • Ricardo Villa-Bellosta, Magda R. Hamczyk
    Pages 119-129

  10. Immunostaining of Macrophages, Endothelial Cells, and Smooth Muscle Cells in the Atherosclerotic Mouse Aorta

    • Prashanthi Menon, Edward A. Fisher
    Pages 131-148

  11. Immunostaining of Lymphocytes in Mouse Atherosclerotic Plaque

    • Anton Gisterå, Daniel F. J. Ketelhuth
    Pages 149-159

  12. Flow Cytometric Analysis of Immune Cells Within Murine Aorta

    • Breanne N. Gjurich, Parésa L. Taghavie-Moghadam, Elena V. Galkina
    Pages 161-175

  13. In Vitro Differentiation of Naïve CD4+ T Cells: A Tool for Understanding the Development of Atherosclerosis

    • Salvador Iborra, Jose M. González-Granado
    Pages 177-189

  14. Quantification of Apoptosis in Mouse Atherosclerotic Lesions

    • Nichola L. Figg, Martin R. Bennett
    Pages 191-199

  15. Quantification of Cellular Proliferation in Mouse Atherosclerotic Lesions

    • José J. Fuster
    Pages 201-210

  16. Quantification of In Vitro Macrophage Cholesterol Efflux and In Vivo Macrophage-Specific Reverse Cholesterol Transport

    • Joan Carles Escolà-Gil, Miriam Lee-Rueckert, David Santos, Lídia Cedó, Francisco Blanco-Vaca, Josep Julve
    Pages 211-233

  17. In Vitro Macrophage Phagocytosis Assay

    • Magda R. Hamczyk, Ricardo Villa-Bellosta, Vicente Andrés
    Pages 235-246

  18. Glucose and Insulin Tolerance Tests in the Mouse

    • Ángela Vinué, Herminia González-Navarro
    Pages 247-254

  19. Wire Myography to Study Vascular Tone and Vascular Structure of Isolated Mouse Arteries

    • Lara del Campo, Mercedes Ferrer
    Pages 255-276

  20. Pressure Myography to Study the Function and Structure of Isolated Small Arteries

    • Olav L. Schjørring, Rune Carlsson, Ulf Simonsen
    Pages 277-295
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Keywords

About this book

This volume reviews available mouse models of atherosclerosis, basic aspects of disease initiation and progression, and the visualization of atherosclerotic lesions with invasive and non-invasive techniques that are widely used both in basic research and in the clinic. The chapters catalogue a wide range of in vitro and in vivo experimental methods used for atherosclerosis research, including thorough protocols and valuable notes based on the authors’ personal wet lab experience. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Methods in Mouse Atherosclerosis will be a valuable working guide for researchers performing mouse-based atherosclerosis studies.

Editors and Affiliations

  • Laboratory of Molecular and Genetic Cardiovascular Pathophysiology, Centro Nacional de Investigaciones Cardiovascular Carlos III (CNIC), Madrid, Spain

    Vicente Andrés, Beatriz Dorado

Bibliographic Information

  • Book Title: Methods in Mouse Atherosclerosis

  • Editors: Vicente Andrés, Beatriz Dorado

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-2929-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2015

  • Hardcover ISBN: 978-1-4939-2928-3Published: 09 October 2015

  • Softcover ISBN: 978-1-4939-4935-9Published: 23 August 2016

  • eBook ISBN: 978-1-4939-2929-0Published: 07 October 2015

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 400

  • Number of Illustrations: 16 b/w illustrations, 58 illustrations in colour

  • Topics: Molecular Medicine, Cardiology, Animal Models

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