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The BAM Complex

Methods and Protocols

  • Book
  • © 2015

Overview

Editors:
  1. Susan Buchanan

    1. National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Laboratory of Molecular Biology, , Bethesda, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. Nicholas Noinaj

    1. Department of Biological Sciences,, Markey Center for Structural Biology, Purdue University, West Lafayette, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1329)

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Table of contents (23 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. The β-Barrel Assembly Machinery Complex

    • Denisse L. Leyton, Matthew J. Belousoff, Trevor Lithgow
    Pages 1-16

  3. Yeast Mitochondria as a Model System to Study the Biogenesis of Bacterial β-Barrel Proteins

    • Thomas Ulrich, Philipp Oberhettinger, Ingo B. Autenrieth, Doron Rapaport
    Pages 17-31

  4. Experimental Methods for Studying the BAM Complex in Neisseria meningitidis

    • Martine P. Bos, Ria Tommassen-van Boxtel, Jan Tommassen
    Pages 33-49

  5. Heat Modifiability of Outer Membrane Proteins from Gram-Negative Bacteria

    • Nicholas Noinaj, Adam J. Kuszak, Susan K. Buchanan
    Pages 51-56

  6. The Role of a Destabilized Membrane for OMP Insertion

    • Ashlee M. Plummer, Dennis Gessmann, Karen G. Fleming
    Pages 57-65

  7. Treponema pallidum in Gel Microdroplets: A Method for Topological Analysis of BamA (TP0326) and Localization of Rare Outer Membrane Proteins

    • Amit Luthra, Arvind Anand, Justin D. Radolf
    Pages 67-75

  8. Analyzing the Role of Periplasmic Folding Factors in the Biogenesis of OMPs and Members of the Type V Secretion System

    • Gustavo Bodelón, Elvira Marín, Luis Ángel Fernández
    Pages 77-110

  9. An In Vitro Assay for Substrate Translocation by FhaC in Liposomes

    • Enguo Fan, Derrick Norell, Matthias Müller
    Pages 111-125

  10. Measuring Cell–Cell Binding Using Flow-Cytometry

    • Zachary C. Ruhe, Christopher S. Hayes, David A. Low
    Pages 127-136

  11. Methods to Characterize Folding and Function of BamA Cross-Link Mutants

    • Adam J. Kuszak, Nicholas Noinaj, Susan K. Buchanan
    Pages 137-147

  12. Small Angle X-ray Scattering (SAXS) Characterization of the POTRA Domains of BamA

    • Pamela Arden Doerner, Marcelo Carlos Sousa
    Pages 149-155

  13. Assessing the Outer Membrane Insertion and Folding of Multimeric Transmembrane β-Barrel Proteins

    • Jack C. Leo, Philipp Oberhettinger, Dirk Linke
    Pages 157-167

  14. The Expression, Purification, and Structure Determination of BamA from E. coli

    • Dongchun Ni, Yihua Huang
    Pages 169-178

  15. Expression and Purification of the Individual Bam Components BamB–E

    • Suraaj Aulakh, Kelly H. Kim, Mark Paetzel
    Pages 179-188

  16. Structure Determination of the BAM Complex Accessory Lipoproteins BamB–E

    • Kornelius Zeth
    Pages 189-202

  17. An In Vitro Assay for Outer Membrane Protein Assembly by the BAM Complex

    • Giselle Roman-Hernandez, Harris D. Bernstein
    Pages 203-213

  18. Identification of BamC on the Surface of E. coli

    • Chaille T. Webb, Trevor Lithgow
    Pages 215-225

  19. Construction and Characterization of an E. coli bamD Depletion Strain

    • Dante P. Ricci
    Pages 227-243

  20. Expression, Purification, and Screening of BamE, a Component of the BAM Complex, for Structural Characterization

    • Mark Jeeves, Pooja Sridhar, Timothy J. Knowles
    Pages 245-258
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Keywords

About this book

This volume is comprised of a collection of experimental protocols for common techniques and strategies used to study the biogenesis of b-barrel outer membrane proteins in Gram-negative bacteria. The BAM Complex: Methods and Protocols guides readers through methods on the function of the BAM complex, the roles played by each of the individual components, the expression and purification of the components, crystallization and structure determination of the components, and how the individual Bam components may assemble into a functional complex. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, The BAM Complex: Methods and Protocols will serve as an invaluable reference for those interested in studying the BAM complex.

Editors and Affiliations

  • National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Laboratory of Molecular Biology, , Bethesda, USA

    Susan Buchanan

  • Department of Biological Sciences,, Markey Center for Structural Biology, Purdue University, West Lafayette, USA

    Nicholas Noinaj

Bibliographic Information

  • Book Title: The BAM Complex

  • Book Subtitle: Methods and Protocols

  • Editors: Susan Buchanan, Nicholas Noinaj

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-2871-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2015

  • Hardcover ISBN: 978-1-4939-2870-5Published: 02 October 2015

  • Softcover ISBN: 978-1-4939-4920-5Published: 23 August 2016

  • eBook ISBN: 978-1-4939-2871-2Published: 01 October 2015

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 283

  • Number of Illustrations: 27 b/w illustrations, 16 illustrations in colour

  • Topics: Protein Science, Membrane Biology

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