Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1333)
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Table of contents (17 protocols)
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Introduction
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Quantification of Persistence
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Single Cell Analysis of Persister Cells
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Identification of Persister Mutants and Genes
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Cellular and Animal Model Systems for Studying Persistence
Keywords
About this book
This volume presents a comprehensive collection of methods that have been instrumental to the current understanding of bacterial persisters. Chapters in the book cover topics ranging from general methods for measuring persister levels in Escherichia coli cultures, protocols for the determination of the persister subpopulation in Candida albicans, quantitative measurements of Type I and Type II persisters using ScanLag, to in vitro and in vivo models for the study of the intracellular activity of antibiotics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Bacterial Persistence: Methods and Protocols brings together the most respected researchers in bacterial persistence whose studies will remain vital to understanding this field for many years to come.
Editors and Affiliations
About the editors
Bibliographic Information
Book Title: Bacterial Persistence
Book Subtitle: Methods and Protocols
Editors: Jan Michiels, Maarten Fauvart
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-2854-5
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2016
Hardcover ISBN: 978-1-4939-2853-8Published: 15 October 2015
Softcover ISBN: 978-1-4939-4904-5Published: 23 August 2016
eBook ISBN: 978-1-4939-2854-5Published: 14 October 2015
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XI, 244
Number of Illustrations: 22 b/w illustrations, 30 illustrations in colour
Topics: Immunology, Bacteriology, Drug Resistance