Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1320)
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Table of contents (22 protocols)
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Front Matter
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Introduction
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Front Matter
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RNA Preparation and Crystallogenesis
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Front Matter
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Data Collection, Phasing and Refinement
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Front Matter
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About this book
This volume includes comprehensive and up-to-date coverage of all nucleic-acid-specific steps used in X-ray crystallography, from macromolecule production to structure determination. Chapters dedicated to RNA preparation and crystallogenesis will be of interest to beginners, while chapters focused on data collection, phasing and refinement will be particularly useful to researchers with a higher level of expertise. Several functional case studies are also presented in the last part of the book. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and thorough, Nucleic Acid Crystallography: Methods and Protocols presents protocols that are aimed at both researchers and students who are interested in the structural biology of DNA or RNA, alone or in complex with proteins or ligands.
Editors and Affiliations
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CNRS Inst de Biol Moléculaire et Cellulaire, Strasbourg, France
Eric Ennifar
About the editor
Bibliographic Information
Book Title: Nucleic Acid Crystallography
Book Subtitle: Methods and Protocols
Editors: Eric Ennifar
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-2763-0
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2016
Hardcover ISBN: 978-1-4939-2762-3Published: 31 July 2015
Softcover ISBN: 978-1-4939-4641-9Published: 19 October 2016
eBook ISBN: 978-1-4939-2763-0Published: 30 July 2015
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XI, 356
Number of Illustrations: 18 b/w illustrations, 98 illustrations in colour
Topics: Nucleic Acid Chemistry