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Molecular Typing of Blood Cell Antigens

  • Book
  • © 2015

Overview

Editors:
  1. Peter Bugert

    1. Medical Faculty Mannheim, German Red Cross Blood Service Baden-Wurttemberg – Hessen, Institute of Transfusion Medicine and Immunology, Heidelberg University, Mannheim, Germany

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1310)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Validation of Genotyping Protocols for Diagnostic Use

    • Paul Metcalfe
    Pages 1-3

  3. High-Resolution Melting Analysis of Single Nucleotide Polymorphisms

    • Carol M. Bruzzone, Clifford J. Steer
    Pages 5-27

  4. High-Speed Droplet-Allele-Specific Polymerase Chain Reaction for Genotyping of Single Nucleotide Polymorphisms

    • Kazuyuki Matsuda, Takayuki Honda
    Pages 29-35

  5. Blood Grouping Based on PCR Methods and Agarose Gel Electrophoresis

    • Ana Maria Sell, Jeane Eliete Laguila Visentainer
    Pages 37-49

  6. Parallel Donor Genotyping for 46 Selected Blood Group and 4 Human Platelet Antigens Using High-Throughput MALDI-TOF Mass Spectrometry

    • Stefan Meyer, Nadine Trost, Beat M. Frey, Christoph Gassner
    Pages 51-70

  7. PCR with Sequence-Specific Primers for Typing of Diallelic Blood Groups

    • Gabriele Rink, Erwin A. Scharberg, Peter Bugert
    Pages 71-81

  8. High-Resolution Melting Analysis for Genotyping Duffy Blood Group Antigens

    • Ewa Łukasik, Kazimiera Waśniowska, Magdalena Grodecka, Edyta Majorczyk, Marcin Czerwiński
    Pages 83-95

  9. Molecular RHD-RHCE Analysis by Multiplex PCR of Short Fluorescent Fragments

    • Yann Fichou, Claude Férec
    Pages 97-104

  10. Microarrays in Blood Group Genotyping

    • Stephanie A. Boccoz, Gaëlle Le Goff, Loïc J. Blum, Christophe A. Marquette
    Pages 105-113

  11. Next-Generation Sequencing for Antenatal Prediction of KEL1 Blood Group Status

    • Klaus Rieneck, Frederik Banch Clausen, Morten Hanefeld Dziegiel
    Pages 115-121

  12. 454-Sequencing™ for the KEL, JR, and LAN Blood Groups

    • Carola Wieckhusen, Peter Bugert
    Pages 123-133

  13. Noninvasive Prenatal Blood Group Genotyping

    • Andrea Doescher, Thomas H. Müller
    Pages 135-147

  14. Genotyping of Human Platelet Antigens by BeadChip Microarray Technology

    • Gerald Bertrand, Fabiana Conti
    Pages 149-165

  15. Sequence-Based Typing for Platelet alloantigens

    • Shun-Chung Pai, Liang-In Lin
    Pages 167-174

  16. Miniaturized Technology for DNA Typing: Cassette PCR

    • Dammika P. Manage, Linda M. Pilarski
    Pages 175-191

  17. Geno- and Phenotyping of Human Neutrophil Antigens

    • Angelika Reil, Jürgen Bux
    Pages 193-203

  18. Allelic Discrimination by TaqMan-PCR for Genotyping of Human Neutrophil Antigens

    • Rudi Steffensen, John Baech, Kaspar R. Nielsen
    Pages 205-212

  19. Novel Approaches and Technologies in Molecular HLA Typing

    • Paul P. J. Dunn
    Pages 213-230

  20. Luminex-Based Methods in High-Resolution HLA Typing

    • Manuela Testi, Marco Andreani
    Pages 231-245
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Keywords

About this book

This volume aims to bring together a variety of protocols useful for DNA-based typing of blood cell antigens. Protocols range from simple approaches with low technical complexity to highly sophisticated modern developments. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Molecular Typing of Blood Cell Antigens summarizes contributions from leading scientist in the field DNA typing for blood cell antigens.

Editors and Affiliations

  • Medical Faculty Mannheim, German Red Cross Blood Service Baden-Wurttemberg – Hessen, Institute of Transfusion Medicine and Immunology, Heidelberg University, Mannheim, Germany

    Peter Bugert

Bibliographic Information

  • Book Title: Molecular Typing of Blood Cell Antigens

  • Editors: Peter Bugert

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-2690-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2015

  • Hardcover ISBN: 978-1-4939-2689-3Published: 30 May 2015

  • Softcover ISBN: 978-1-4939-5587-9Published: 09 October 2016

  • eBook ISBN: 978-1-4939-2690-9Published: 29 May 2015

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 261

  • Number of Illustrations: 15 b/w illustrations, 46 illustrations in colour

  • Topics: Gene Function

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