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Stem Cell Renewal and Cell-Cell Communication

Methods and Protocols

  • Book
  • © 2015

Overview

Editors:
  1. Kursad Turksen

    1. Ottawa Hospital Research Institute, Ottawa, Canada

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  • Includes cutting-edge methods and protocols covering the intersection between cell-cell interactions and stem cell renewal
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1212)

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Table of contents (15 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. Laboratory Control and Basic Culture Protocols for Stem Cell Self-Renewal

    • Jeong Mook Lim, Yoon Mi Jo, Ji Yeon Ahn
    Pages 1-20

  3. Advanced Fixation for Transmission Electron Microscopy Unveils Special Extracellular Matrix Within the Renal Stem/Progenitor Cell Niche

    • Will W. Minuth, Lucia Denk
    Pages 21-37

  4. Multiplex Immunoassays for Quantification of Cytokines, Growth Factors, and Other Proteins in Stem Cell Communication

    • Ivona Valekova, Helena Kupcova Skalnikova, Karla Jarkovska, Jan Motlik, Hana Kovarova
    Pages 39-63

  5. Porous Membrane Culture Method for Expansion of Human Pluripotent Stem Cells

    • Jin-Su Kim, Seung-Taeh Hwang, Soo-Hong Lee
    Pages 65-72

  6. Tuning Differentiation Signals for Efficient Propagation and In Vitro Validation of Rat Embryonic Stem Cell Cultures

    • Stephen Meek, Linda Sutherland, Tom Burdon
    Pages 73-85

  7. Generation, Expansion, and Differentiation of Human Pluripotent Stem Cell (hPSC) Derived Neural Progenitor Cells (NPCs)

    • David A. Brafman
    Pages 87-102

  8. Isolation, Long-Term Expansion, and Differentiation of Murine Neural Stem Cells

    • Alexandra Bizy, Sacri R. Ferrón
    Pages 103-112

  9. Generation, Expansion, and Differentiation of Cardiovascular Progenitor Cells from Human Pluripotent Stem Cells

    • Nan Cao, He Liang, Huang-Tian Yang
    Pages 113-125

  10. A Practical Guide for the Isolation and Maintenance of Stem Cells from Tendon

    • Pauline Po Yee Lui
    Pages 127-140

  11. A Human Colonic Crypt Culture System to Study Regulation of Stem Cell-Driven Tissue Renewal and Physiological Function

    • Alyson Parris, Mark R. Williams
    Pages 141-161

  12. Ca2+ Handling in Mouse Embryonic Stem Cell-Derived Cardiomyocytes

    • Wenjie Wei, Jianbo Yue
    Pages 163-169

  13. Potential Application of Extracellular Vesicles of Human Adipose Tissue-Derived Mesenchymal Stem Cells in Alzheimer’s Disease Therapeutics

    • Takeshi Katsuda, Katsuyuki Oki, Takahiro Ochiya
    Pages 171-181

  14. Application of Fluid Mechanical Force to Embryonic Sources of Hemogenic Endothelium and Hematopoietic Stem Cells

    • Nan Li, Miguel F. Diaz, Pamela L. Wenzel
    Pages 183-193

  15. Electrophysiological Recordings from Neuroepithelial Stem Cells

    • Masayuki Yamashita
    Pages 195-200

  16. In Vivo Stem Cell Transplantation Using Reduced Cell Numbers

    • Takeo W. Tsutsui
    Pages 201-207

  17. Back Matter

    Pages 209-210
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Keywords

About this book

This volume examines cell-cell interactions and stem cell renewal, two topics that are now inexorably linked as science strives to understand the stem cell niche and its function. Gathering a number of representative protocols, this detailed collection promises to provide readers with approaches for studying these complimentary aspects of stem cells. Written for the highly successful Methods in Molecular Biology series, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Practical and reliable, Stem Cell Renewal and Cell-Cell Communication: Methods and Protocols will aid researchers in using these methods to advance their own studies.

Editors and Affiliations

  • Ottawa Hospital Research Institute, Ottawa, Canada

    Kursad Turksen

Bibliographic Information

  • Book Title: Stem Cell Renewal and Cell-Cell Communication

  • Book Subtitle: Methods and Protocols

  • Editors: Kursad Turksen

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-2590-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2015

  • Hardcover ISBN: 978-1-4939-2589-6Published: 20 May 2015

  • Softcover ISBN: 978-1-4939-4939-7Published: 01 November 2016

  • eBook ISBN: 978-1-4939-2590-2Published: 18 May 2015

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 210

  • Number of Illustrations: 20 b/w illustrations, 24 illustrations in colour

  • Topics: Cell Biology

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