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Cardiomyocytes

Methods and Protocols

  • Book
  • © 2015

Overview

Editors:
  1. Gary R. Skuse

    1. Rochester Institute of Technology, Rochester, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. Maureen C. Ferran

    1. Rochester Institute of Technology, Rochester, USA

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols in cardiomyocyte research
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1299)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. Generating Primary Cultures of Murine Cardiac Myocytes and Cardiac Fibroblasts to Study Viral Myocarditis

    • Barbara Sherry
    Pages 1-16

  3. Enrichment of Cardiomyocytes in Primary Cultures of Murine Neonatal Hearts

    • Sreejit Parameswaran, Rajalakshmi Santhakumar, Prasanna Vidyasekar, Rama S. Verma
    Pages 17-25

  4. Deep Sequencing of Cardiac MicroRNA-mRNA Interactomes in Clinical and Experimental Cardiomyopathy

    • Scot J. Matkovich, Gerald W. Dorn II
    Pages 27-49

  5. Next-Generation Sequencing Technology in the Genetics of Cardiovascular Disease

    • Cecilia Vecoli
    Pages 51-64

  6. Computational Cardiac Electrophysiology: Implementing Mathematical Models of Cardiomyocytes to Simulate Action Potentials of the Heart

    • Michael M. Bell, Elizabeth M. Cherry
    Pages 65-74

  7. Methods of Myofibrillogenesis Modeling

    • Nancy K. Drew, Anna Grosberg
    Pages 75-91

  8. Using the Mechanical Bidomain Model to Analyze the Biomechanical Behavior of Cardiomyocytes

    • Bradley J. Roth
    Pages 93-102

  9. Fabrication of a Myocardial Patch with Cells Differentiated from Human-Induced Pluripotent Stem Cells

    • Lei Ye, Joydeep Basu, Jianyi Zhang
    Pages 103-114

  10. Efficient Differentiation of Cardiomyocytes from Human Pluripotent Stem Cells with Growth Factors

    • Rajneesh Jha, Ren-He Xu, Chunhui Xu
    Pages 115-131

  11. Isolation, Culturing, and Characterization of Cardiac Muscle Cells from Nonhuman Primate Heart Tissue

    • Steven M. Hoynowski, John W. Ludlow
    Pages 133-143

  12. Mouse Embryonic Stem Cell-Derived Cardiac Myocytes in a Cell Culture Dish

    • Carley Glass, Reetu Singla, Anshu Arora, Dinender K. Singla
    Pages 145-152

  13. Cryopreservation of Neonatal Cardiomyocytes

    • Adam C. Vandergriff, M. Taylor Hensley, Ke Cheng
    Pages 153-160

  14. Evaluation of Sarcomeric Organization in Human Pluripotent Stem Cell-Derived Cardiomyocytes

    • Chrishan J. A. Ramachandra, Winston Shim
    Pages 161-166

  15. Electrotonic Coupled Metabolic Purification of Chick Cardiomyocytes

    • Winston Shim, Haiyang Yu, K. P. Myu Mai Ja, Muhammad Parasuram, Kee Pah Lim, Philip Wong
    Pages 167-175

  16. Gene Transfer into Cardiac Myocytes

    • Sarah E. Lang, Margaret V. Westfall
    Pages 177-190

  17. Analysis of 4D Myocardial Wall Motion During Early Stages of Chick Heart Development

    • Madeline Midgett, Sandra Rugonyi
    Pages 191-212

  18. Back Matter

    Pages 213-216
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Keywords

About this book

This volume has been assembled for scientists interested in basic and applied biomedical research directed toward understanding the development, genetics and function of cardiomyocytes. The methods and protocols contained within it address cell culture techniques, cardiomyocyte differentiation and redifferentiation, experimental induction of cardiomyopathies, introducing genes into cardiomyocytes, genomic approaches to the understanding cardiomyocytes, cryopreservation of neonatal cardiomyocytes and modeling of cardiomyocyte function. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.  

Practical and current, Cardiomyocytes: Methods and Protocols explores complimentary areas of cardiomyocyte science that, taken together, can inform individuals with a broad range of interests.

Editors and Affiliations

  • Rochester Institute of Technology, Rochester, USA

    Gary R. Skuse, Maureen C. Ferran

Bibliographic Information

  • Book Title: Cardiomyocytes

  • Book Subtitle: Methods and Protocols

  • Editors: Gary R. Skuse, Maureen C. Ferran

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-2572-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2015

  • Hardcover ISBN: 978-1-4939-2571-1Published: 03 April 2015

  • Softcover ISBN: 978-1-4939-4754-6Published: 05 October 2016

  • eBook ISBN: 978-1-4939-2572-8Published: 02 April 2015

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 216

  • Number of Illustrations: 8 b/w illustrations, 28 illustrations in colour

  • Topics: Cell Biology

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