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  • © 2015

Mycobacteria Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1285)

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Table of contents (25 protocols)

  1. Front Matter

    Pages i-xii
  2. Whole-Genome Sequencing for Comparative Genomics and De Novo Genome Assembly

    • Andrej Benjak, Claudia Sala, Ruben C. Hartkoorn
    Pages 1-16
  3. RNA Sequencing for Transcript 5′-End Mapping in Mycobacteria

    • Scarlet S. Shell, Michael R. Chase, Thomas R. Ioerger, Sarah M. Fortune
    Pages 31-45
  4. Fractionation and Analysis of Mycobacterial Proteins

    • Megan C. Lucas, Lisa M. Wolfe, Rachel M. Hazenfield, Jade Kurihara, Nicole A. Kruh-Garcia, John Belisle et al.
    Pages 47-75
  5. Lipid and Lipoarabinomannan Isolation and Characterization

    • Marie-Antoinette Lanéelle, Jérôme Nigou, Mamadou Daffé
    Pages 77-103
  6. Metabolomics of Mycobacterium tuberculosis

    • Madhumitha Nandakumar, Gareth A. Prosser, Luiz Pedro S. de Carvalho, Kyu Rhee
    Pages 105-115
  7. Electroporation of Mycobacteria

    • Renan Goude, David M. Roberts, Tanya Parish
    Pages 117-130
  8. Targeted Gene Knockout and Essentiality Testing by Homologous Recombination

    • Krishnamoorthy Gopinath, Digby F. Warner, Valerie Mizrahi
    Pages 131-149
  9. Construction of Conditional Knockdown Mutants in Mycobacteria

    • Dirk Schnappinger, Kathryn M. O’Brien, Sabine Ehrt
    Pages 151-175
  10. Mycobacterial Recombineering

    • Kenan C. Murphy, Kadamba Papavinasasundaram, Christopher M. Sassetti
    Pages 177-199
  11. Genetic Dissection of Mycobacterial Biofilms

    • Anil K. Ojha, William R. Jacobs Jr., Graham F. Hatfull
    Pages 215-226
  12. Measuring Efflux and Permeability in Mycobacteria

    • Liliana Rodrigues, Miguel Viveiros, José A. Aínsa
    Pages 227-239
  13. Antimicrobial Susceptibility Testing for Mycobacterium sp.

    • Delia Blanco-Ruano, David M. Roberts, Ruben Gonzalez-Del-Rio, Daniel Álvarez, Mª José Rebollo, Esther Pérez-Herrán et al.
    Pages 257-268

About this book

Updated and revised, this thorough volume provides a selection of the newest methods, as well as some of the basic methods required for a mycobacterial research laboratory. Mycobacteria Protocols, Third Edition guides readers through fractionation and analysis of macromolecules, from nucleic acids to proteins, complex lipids, and metabolites. Detailed and comprehensive protocols are provided for protein and lipid/glycolipid analysis using well-established methods; these are now complemented by a metabolomics chapter in which the complement of metabolites can be profiled. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Authoritative and up-to-date, Mycobacteria Protocols, Third Edition will be a resource both to those working in the field and to newcomers.

Editors and Affiliations

  • Infectious Disease Research Inst (IDRI), Seattle, USA

    Tanya Parish, David M. Roberts

Bibliographic Information

  • Book Title: Mycobacteria Protocols

  • Editors: Tanya Parish, David M. Roberts

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-2450-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2015

  • Hardcover ISBN: 978-1-4939-2449-3Published: 17 March 2015

  • Softcover ISBN: 978-1-4939-5582-4Published: 05 October 2016

  • eBook ISBN: 978-1-4939-2450-9Published: 16 March 2015

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 3

  • Number of Pages: XII, 416

  • Number of Illustrations: 51 b/w illustrations, 37 illustrations in colour

  • Topics: Microbiology

Buy it now

Buying options

eBook USD 109.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 139.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access