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  • © 2015

Stem Cells and Good Manufacturing Practices

Methods, Protocols, and Regulations

Editors:

  • Includes cutting-edge methods and protocols for the study of good manufacturing practices and stem cells
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1283)

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Table of contents (18 protocols)

  1. Front Matter

    Pages i-xi
  2. Scalable Expansion of Human-Induced Pluripotent Stem Cells in Xeno-Free Microcarriers

    • Sara M. Badenes, Tiago G. Fernandes, Carlos A. V. Rodrigues, Maria Margarida Diogo, Joaquim M. S. Cabral
    Pages 23-29
  3. Derivation of GMP-Compliant Integration-Free hiPSCs Using Modified mRNAs

    • Jens Durruthy Durruthy, Vittorio Sebastiano
    Pages 31-42
  4. Labeling Pluripotent Stem Cell-Derived Neural Progenitors with Iron Oxide Particles for Magnetic Resonance Imaging

    • Sébastien Sart, Fabian Calixto Bejarano, Yuanwei Yan, Samuel C. Grant, Yan Li
    Pages 43-52
  5. Xeno-Free Culture of Human Periodontal Ligament Stem Cells

    • Oriana Trubiani, Francesca Diomede
    Pages 87-92
  6. GMP-Compliant Human Adipose Tissue-Derived Mesenchymal Stem Cells for Cellular Therapy

    • Hamid-Reza Aghayan, Parisa Goodarzi, Babak Arjmand
    Pages 93-107
  7. cGMP-Compliant Transportation Conditions for a Prompt Therapeutic Use of Marrow Mesenchymal Stromal/Stem Cells

    • Elena Veronesi, Jorge Sans Burns, Alba Murgia, Olivia Candini, Valeria Rasini, Ilenia Mastrolia et al.
    Pages 109-122
  8. GMP-Grade Human Fetal Liver-Derived Mesenchymal Stem Cells for Clinical Transplantation

    • Bagher Larijani, Hamid-Reza Aghayan, Parisa Goodarzi, Babak Arjmand
    Pages 123-136
  9. Purification of Human Induced Pluripotent Stem Cell-Derived Neural Precursors Using Magnetic Activated Cell Sorting

    • Gonçalo M. C. Rodrigues, Tiago G. Fernandes, Carlos A. V. Rodrigues, Joaquim M. S. Cabral, Maria Margarida Diogo
    Pages 137-145
  10. Scalable Ex Vivo Expansion of Human Mesenchymal Stem/Stromal Cells in Microcarrier-Based Stirred Culture Systems

    • Joana G. Carmelo, Ana Fernandes-Platzgummer, Joaquim M. S. Cabral, Cláudia Lobato da Silva
    Pages 147-159
  11. Culture of Human Limbal Epithelial Stem Cells on Tenon’s Fibroblast Feeder-Layers: A Translational Approach

    • Gaia Scafetta, Camilla Siciliano, Giacomo Frati, Elena De Falco
    Pages 187-198
  12. Bioreactor Expansion of Human Mesenchymal Stem Cells According to GMP Requirements

    • Christiane L. Elseberg, Denise Salzig, Peter Czermak
    Pages 199-218
  13. Back Matter

    Pages 219-220

About this book

This volume collects a series of protocols describing the kinds of infrastructures, training, and standard operating procedures currently available to actualize the potential of stem cells for regenerative therapies. Stem Cells and Good Manufacturing Practices: Methods, Protocols, and Regulations pulls together key GMP techniques from laboratories around the world. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Inclusive and authoritative, Stem Cells and Good Manufacturing Practices: Methods, Protocols, and Regulations will be an invaluable resource to both basic and clinical practitioners in stem cell biology.

Editors and Affiliations

  • Ottawa Hospital Research Institute, Ottawa, Canada

    Kursad Turksen

Bibliographic Information

  • Book Title: Stem Cells and Good Manufacturing Practices

  • Book Subtitle: Methods, Protocols, and Regulations

  • Editors: Kursad Turksen

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-2435-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2015

  • Hardcover ISBN: 978-1-4939-2434-9Published: 14 February 2015

  • Softcover ISBN: 978-1-4939-4940-3Published: 22 October 2016

  • eBook ISBN: 978-1-4939-2435-6Published: 13 February 2015

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 220

  • Number of Illustrations: 34 illustrations in colour

  • Topics: Cell Biology, Stem Cells

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access