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  • © 2015

Mitochondrial Medicine

Volume I, Probing Mitochondrial Function

  • Includes cutting-edge methods and protocols for the assessment of mitochondrial function
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1264)

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Softcover Book USD 119.99
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  • Dispatched in 3 to 5 business days
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Hardcover Book USD 169.99
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  • Durable hardcover edition
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Table of contents (38 protocols)

  1. Front Matter

    Pages i-xxv
  2. Preparation of “Functional” Mitochondria: A Challenging Business

    • Stefan Lehr, Sonja Hartwig, Jorg Kotzka
    Pages 1-8
  3. Isolation and Quality Control of Functional Mitochondria

    • Sonja Hartwig, Jorg Kotzka, Stefan Lehr
    Pages 9-23
  4. Fluorescence Analysis of Single Mitochondria with Nanofluidic Channels

    • Ted Pham, Katayoun Zand, Douglas Wallace, Peter Burke
    Pages 35-46
  5. Optical Microwell Arrays for Large-Scale Studies of Single Mitochondria Metabolic Responses

    • Venkata Suresh Vajrala, Emmanuel Suraniti, Bertrand Goudeau, Neso Sojic, Stéphane Arbault
    Pages 47-58
  6. Deep Resequencing of Mitochondrial DNA

    • Brendan A. I. Payne, Kristian Gardner, Jonathan Coxhead, Patrick F. Chinnery
    Pages 59-66
  7. Single-Cell Analysis of Mitochondrial DNA

    • Brendan A. I. Payne, Lynsey Cree, Patrick F. Chinnery
    Pages 67-76
  8. Rapid Mitochondrial DNA Isolation Method for Direct Sequencing

    • Wilber Quispe-Tintaya, Ryan R. White, Vasily N. Popov, Jan Vijg, Alexander Y. Maslov
    Pages 89-95
  9. Analysis of Mitochondrial DNA and RNA Integrity by a Real-Time qPCR-Based Method

    • Wei Wang, Ying Esbensen, Katja Scheffler, Lars Eide
    Pages 97-106
  10. Mitochondria-Targeted RNA Import

    • Geng Wang, Eriko Shimada, Mahta Nili, Carla M. Koehler, Michael A. Teitell
    Pages 107-116
  11. Simultaneous Quantification of Mitochondrial ATP and ROS Production

    • Liping Yu, Brian D. Fink, William I. Sivitz
    Pages 149-159
  12. Live-Cell Assessment of Mitochondrial Reactive Oxygen Species Using Dihydroethidine

    • Marleen Forkink, Peter H. G. M. Willems, Werner J. H. Koopman, Sander Grefte
    Pages 161-169
  13. Time-Resolved Spectrometry of Mitochondrial NAD(P)H Fluorescence and Its Applications for Evaluating the Oxidative State in Living Cells

    • Julia Horilova, Hauke Studier, Zuzana Nadova, Pavol Miskovsky, Dusan Chorvat Jr., Alzbeta Marcek Chorvatova
    Pages 183-193

About this book

This expert volume covers an interdisciplinary and rapidly growing area of biomedical research comprising genetic, biochemical, pathological, and clinical studies aimed at the diagnosis and therapy of human diseases which are either caused by or associated with mitochondrial dysfunction. It dedicates itself to showcasing the tremendous efforts and the progress that has been made over the last decades in developing techniques and protocols for probing, imaging, and manipulating mitochondrial functions. Mitochondrial Medicine: Volume I, Probing Mitochondrial Function focuses on methods being used for the assessment of mitochondrial function under physiological conditions as well as in healthy isolated mitochondria. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and practical, Mitochondrial Medicine provides an essential source of know-how and inspiration to all researchers who are fascinated by this tiny organelle that seems so clearly to control the life and death of a single cell and whole organisms alike.

Editors and Affiliations

  • Midwestern University, Glendale, USA

    Volkmar Weissig

  • ISANH, Paris, France

    Marvin Edeas

Bibliographic Information

  • Book Title: Mitochondrial Medicine

  • Book Subtitle: Volume I, Probing Mitochondrial Function

  • Editors: Volkmar Weissig, Marvin Edeas

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-2257-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2015

  • Hardcover ISBN: 978-1-4939-2256-7Published: 29 January 2015

  • Softcover ISBN: 978-1-4939-5572-5Published: 05 October 2016

  • eBook ISBN: 978-1-4939-2257-4Published: 28 January 2015

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XXV, 480

  • Number of Illustrations: 42 b/w illustrations, 66 illustrations in colour

  • Topics: Molecular Medicine, Cell Culture

Buy it now

Buying options

Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access