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Neuronal Cell Death

Methods and Protocols

  • Book
  • © 2015

Overview

Editors:
  1. Laura Lossi

    1. Dept. of Veterinary Sciences, University of Torino, Grugliasco, Italy

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    You can also search for this editor in PubMed   Google Scholar

  2. Adalberto Merighi

    1. Dept. of Veterinary Sciences, University of Torino, Grugliasco, Italy

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1254)

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Table of contents (26 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Neuronal Cell Death: An Overview of Its Different Forms in Central and Peripheral Neurons

    • Laura Lossi, Claudia Castagna, Adalberto Merighi
    Pages 1-18

  3. Molecular, Structural, Functional, and Genomic Changes in Dying Neurons

    1. Front Matter

      Pages 19-19
      Download chapter PDF

    2. Cell Volume Regulation Monitored with Combined Epifluorescence and Digital Holographic Microscopy

      • Nicolas Pavillon, Pierre Marquet
      Pages 21-32

    3. Flow Cytometric Analysis of DNA Synthesis and Apoptosis in Central Nervous System Using Fresh Cell Nuclei

      • Noelia López-Sánchez, José M. Frade
      Pages 33-42

    4. Nuclear Signs of Pre-neurodegeneration

      • Fernando C. Baltanás, Jorge Valero, Jose Ramón Alonso, Maria Teresa Berciano, Miguel Lafarga
      Pages 43-54

    5. Multi-parametric O2 Imaging in Three-Dimensional Neural Cell Models with the Phosphorescent Probes

      • Ruslan I. Dmitriev, Dmitri B. Papkovsky
      Pages 55-71

    6. Calcium Imaging in Neuron Cell Death

      • María Calvo, Carlos Villalobos, Lucía Núñez
      Pages 73-85

    7. Monitoring Mitochondrial Membranes Permeability in Live Neurons and Mitochondrial Swelling Through Electron Microscopy Analysis

      • Macarena S. Arrázola, Nibaldo C. Inestrosa
      Pages 87-97

    8. Real-Time Visualization of Caspase-3 Activation by Fluorescence Resonance Energy Transfer (FRET)

      • Silvia Alasia, Carolina Cocito, Adalberto Merighi, Laura Lossi
      Pages 99-113

    9. Design and Cloning of Short Hairpin RNAs (shRNAs) into a Lentiviral Silencing Vector to Study the Function of Selected Proteins in Neuronal Apoptosis

      • Nadia Canu
      Pages 115-128

    10. Genomic Analysis Using Affymetrix Standard Microarray GeneChips (169 Format) in Degenerate Murine Retina

      • Sook Hyun Chung, Weiyong Shen, Mark Gillies
      Pages 129-140

    11. Genomic Analysis of Transcriptional Changes Underlying Neuronal Apoptosis

      • Sebastiano Cavallaro
      Pages 141-151

    12. High-Throughput Cell Death Assays

      • Matthew E. Pamenter, Gabriel G. Haddad
      Pages 153-163

  4. Cell Death in Neuropathology and Experimental Neuropathology

    1. Front Matter

      Pages 165-165
      Download chapter PDF

    2. Staining of Dead Neurons by the Golgi Method in Autopsy Material

      • Stavros J. Baloyannis
      Pages 167-179

    3. Image Analysis Algorithms for Immunohistochemical Assessment of Cell Death

      • Stan Krajewski, Jeffrey Wang, Tashmia Khan, Jonathan Liu, Chia-Hung Sze, Maryla Krajewska
      Pages 181-196

    4. In Vitro Oxygen-Glucose Deprivation to Study Ischemic Cell Death

      • Carla I. Tasca, Tharine Dal-Cim, Helena Cimarosti
      Pages 197-210

    5. Laser Microbeam Targeting of Single Nerve Axons in Cell Culture

      • Nicholas Hyun, Linda Z. Shi, Michael W. Berns
      Pages 211-226

    6. Real-Time Imaging of Retinal Cell Apoptosis by Confocal Scanning Laser Ophthalmoscopy

      • Eduardo M. Normando, Mohammad H. Dehabadi, Li Guo, Lisa A. Turner, Gaia Pollorsi, M. Francesca Cordeiro
      Pages 227-237
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Keywords

About this book

This volume represents a valuable and readily reproducible collection of established and emerging techniques for neuronal cell death research. Conveniently divided into four parts, sections cover a series of techniques for the molecular, structural, functional and genomic characterization of dying neurons, a number of protocols that are of primary interest in neuropathology and in experimental neuropathology, a series of gene engineering techniques to obtain and manipulate neuronal stem cells and progenitors, to prepare HSV-1 vectors for the gene therapy, and to CNS transplantation of bone marrow stem cells, and finally, some very interesting protocols for the study of cell death in non-mammalian models. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Neuronal Cell Death: Methods and Protocols seeks to serve a large audience of scientists that are currently active in the field or are willing to enter such an exciting and still expanding area of neurobiology.

Editors and Affiliations

  • Dept. of Veterinary Sciences, University of Torino, Grugliasco, Italy

    Laura Lossi, Adalberto Merighi

Bibliographic Information

  • Book Title: Neuronal Cell Death

  • Book Subtitle: Methods and Protocols

  • Editors: Laura Lossi, Adalberto Merighi

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-2152-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2015

  • Hardcover ISBN: 978-1-4939-2151-5Published: 28 November 2014

  • Softcover ISBN: 978-1-4939-4464-4Published: 23 August 2016

  • eBook ISBN: 978-1-4939-2152-2Published: 27 November 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 396

  • Number of Illustrations: 34 b/w illustrations, 48 illustrations in colour

  • Topics: Neurosciences, Apoptosis

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