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Mitochondrial Regulation

Methods and Protocols

  • Book
  • © 2015

Overview

Editors:
  1. Carlos M. Palmeira

    1. Department of Life Sciences and Center for Neurosciences and Cell Biology, University of Coimbra, Coimbra, Portugal

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  2. Anabela P. Rolo

    1. Department of Biology and Center for Neurosciences and Cell Biology, University of Aveiro and University of Coimbra, Aveiro and Coimbra, Portugal

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1241)

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Table of contents (15 protocols)

  1. Front Matter

    Pages i-xi
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  2. Isolation of Crude Mitochondrial Fraction from Cells

    • Mariusz R. Wieckowski, Lech Wojtczak
    Pages 1-8

  3. Isolation of Mitochondria from Liver and Extraction of Total RNA and Protein: Analyses of MicroRNA and Protein Expressions

    • Pedro M. Borralho, Clifford J. Steer, Cecília M. P. Rodrigues
    Pages 9-22

  4. PCR Based Determination of Mitochondrial DNA Copy Number in Multiple Species

    • John P. Rooney, Ian T. Ryde, Laurie H. Sanders, Evan. H. Howlett, Meryl D. Colton, Kaylyn E. Germ et al.
    Pages 23-38

  5. NAD+ Content and Its Role in Mitochondria

    • Wei Li, Anthony A. Sauve
    Pages 39-48

  6. Measuring PGC-1α and Its Acetylation Status in Mouse Primary Myotubes

    • Ana P. Gomes, David A. Sinclair
    Pages 49-57

  7. Measurement of Mitochondrial Oxygen Consumption Rates in Mouse Primary Neurons and Astrocytes

    • Sofia M. Ribeiro, Alfredo Giménez-Cassina, Nika N. Danial
    Pages 59-69

  8. Determination of Oxidative Phosphorylation Complexes Activities

    • João S. Teodoro, Carlos M. Palmeira, Anabela P. Rolo
    Pages 71-84

  9. Histoenzymatic Methods for Visualization of the Activity of Individual Mitochondrial Respiratory Chain Complexes in the Muscle Biopsies from Patients with Mitochondrial Defects

    • Agnieszka Karkucinska-Wieckowska, Maciej Pronicki, Mariusz R. Wieckowski
    Pages 85-93

  10. A Method Aimed at Assessing the Functional Consequences of the Supramolecular Organization of the Respiratory Electron Transfer Chain by Time-Resolved Studies

    • Fabrice Rappaport
    Pages 95-109

  11. BN-PAGE-Based Approach to Study Thyroid Hormones and Mitochondrial Function

    • Elena Silvestri, Assunta Lombardi, Federica Cioffi, Fernando Goglia
    Pages 111-122

  12. Detection of UCP1 Protein and Measurements of Dependent GDP-Sensitive Proton Leak in Non-phosphorylating Thymus Mitochondria

    • Kieran J. Clarke, Audrey M. Carroll, Gemma O’Brien, Richard K. Porter
    Pages 123-135

  13. Exploring Liver Mitochondrial Function by 13C-Stable Isotope Breath Tests: Implications in Clinical Biochemistry

    • Ignazio Grattagliano, Leonilde Bonfrate, Michele Lorusso, Luigi Castorani, Ornella de Bari, Piero Portincasa
    Pages 137-152

  14. Following Mitochondria Dynamism: Confocal Analysis of the Organelle Morphology

    • Francesca R. Mariotti, Mauro Corrado, Silvia Campello
    Pages 153-161

  15. Analysis of Pro-apoptotic Protein Trafficking to and from Mitochondria

    • Ignacio Vega-Naredo, Teresa Cunha-Oliveira, Teresa L. Serafim, Vilma A. Sardao, Paulo J. Oliveira
    Pages 163-180

  16. Mitophagy and Mitochondrial Balance

    • Simone Patergnani, Paolo Pinton
    Pages 181-194

  17. Back Matter

    Pages 195-201
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Keywords

About this book

In this volume expert researchers in the field detail the many methods for further research into regulation of mitochondrial function. Chapters focus on mitochondria with other cellular components, discussing how these interactions influence the dynamics of mitochondrial structure and biogenesis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Mitochondrial Regulation: Methods and Protocols seeks to aid advanced undergraduates, graduates, postgraduates, and beginning researchers in the areas of molecular and cellular biology, biochemistry and bioenergetics.

Editors and Affiliations

  • Department of Life Sciences and Center for Neurosciences and Cell Biology, University of Coimbra, Coimbra, Portugal

    Carlos M. Palmeira

  • Department of Biology and Center for Neurosciences and Cell Biology, University of Aveiro and University of Coimbra, Aveiro and Coimbra, Portugal

    Anabela P. Rolo

Bibliographic Information

  • Book Title: Mitochondrial Regulation

  • Book Subtitle: Methods and Protocols

  • Editors: Carlos M. Palmeira, Anabela P. Rolo

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-1875-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2015

  • Hardcover ISBN: 978-1-4939-1874-4Published: 11 October 2014

  • Softcover ISBN: 978-1-4939-4612-9Published: 23 August 2016

  • eBook ISBN: 978-1-4939-1875-1Published: 11 October 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 201

  • Number of Illustrations: 12 b/w illustrations, 26 illustrations in colour

  • Topics: Cell Biology

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