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Apoptosis and Cancer

Methods and Protocols

  • Book
  • © 2015

Overview

Editors:
  1. Gil Mor

    1. Department of Obstetrics, Gynecology, and Reproductive Sciences,, Yale University, New Haven, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. Ayesha B. Alvero

    1. Department of Obstetrics, Gynecology, and Reproductive Sciences,, Yale University, New Haven, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1219)

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Table of contents (15 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Caspase-3 Activation Is a Critical Determinant of Genotoxic Stress-Induced Apoptosis

    • Gaurav S. Choudhary, Sayer Al-harbi, Alexandru Almasan
    Pages 1-9

  3. Flow Cytometry Enumeration of Apoptotic Cancer Cells by Apoptotic Rate

    • David Diaz, Alfredo Prieto, Eduardo Reyes, Hugo Barcenilla, Jorge Monserrat, Melchor Alvarez-Mon
    Pages 11-20

  4. “Multiplexed Viability, Cytotoxicity, and Caspase Activity Assays”

    • Andrew L. Niles, Terry L. Riss
    Pages 21-33

  5. A Multiplexed Method for Kinetic Measurements of Apoptosis and Proliferation Using Live-Content Imaging

    • Katherine Artymovich, Daniel M. Appledorn
    Pages 35-42

  6. Detection of End-Stage Apoptosis by ApopTag® TUNEL Technique

    • Chandra Mohan, Kevin Long, Manpreet Mutneja, Jun Ma
    Pages 43-56

  7. Detection and Quantification of Apoptosis in Primary Cells Using Taqman® Protein Assay

    • Christina Pfister, Heike Pfrommer, Marcos S. Tatagiba, Florian Roser
    Pages 57-73

  8. Detection of p53 Protein Aggregation in Cancer Cell Lines and Tumor Samples

    • Yang Yang-Hartwich, Jamie Bingham, Francesca Garofalo, Ayesha B. Alvero, Gil Mor
    Pages 75-86

  9. Detection of p53 Protein Transcriptional Activity by Chromatin Immunoprecipitation

    • Yang Yang-Hartwich, Emily Romanoff, Jamie Bingham, Ayesha B. Alvero, Gil Mor
    Pages 87-93

  10. Homogeneous, Bioluminescent Proteasome Assays

    • Martha A. O’Brien, Richard A. Moravec, Terry L. Riss, Robert F. Bulleit
    Pages 95-114

  11. Laser Capture Microdissection for Gene Expression Analysis

    • Mallikarjun Bidarimath, Andrew K. Edwards, Chandrakant Tayade
    Pages 115-137

  12. Using the Peggy Simple Western System for Fine Needle Aspirate Analysis

    • Erik T. Gentalen, John M. Proctor
    Pages 139-155

  13. Analysis of Autophagosome Formation Using Lentiviral Biosensors for Live Fluorescent Cellular Imaging

    • Kevin Long, Chandra Mohan, Janet Anderl, Karyn Huryn-Selvar, Haizhen Liu, Kevin Su et al.
    Pages 157-169

  14. Optical Imaging of Ovarian Cancer Using HER-2 Affibody Conjugated Nanoparticles

    • Minati Satpathy, Rafal Zielinski, Ilya Lyakhov, Lily Yang
    Pages 171-185

  15. Measuring Cardiac Autophagic Flux In Vitro and In Vivo

    • Michael A. Gurney, Chengqun Huang, Jennifer M. Ramil, Nandini Ravindran, Allen M. Andres, Jon Sin et al.
    Pages 187-197

  16. PET Imaging for Tyrosine Kinase Inhibitor (TKI) Biodistribution in Mice

    • Hiroshi Fushiki, Yoshihiro Murakami, Sosuke Miyoshi, Shintaro Nishimura
    Pages 199-206

  17. Back Matter

    Pages 207-208
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Keywords

About this book

In Apoptosis and Cancer: Methods and Protocols, Second Edition, expert researches in the field detail the performance of molecular and cellular biology techniques for studying and detecting the activation of the apoptotic pathway. Chapters focus on assays developed to detect its activation not only in vitro but also in vivo, optimized multiplex analysis, medium- to high-throughput screens, and the cellular process. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Apoptosis and Cancer: Methods and Protocols, Second Edition aids scientists as a stand-alone resource for the execution and analysis of the described protocols and as a reference for the study and detection of apoptosis within and outside the area of cancer research.

Editors and Affiliations

  • Department of Obstetrics, Gynecology, and Reproductive Sciences,, Yale University, New Haven, USA

    Gil Mor, Ayesha B. Alvero

Bibliographic Information

  • Book Title: Apoptosis and Cancer

  • Book Subtitle: Methods and Protocols

  • Editors: Gil Mor, Ayesha B. Alvero

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-1661-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2015

  • Hardcover ISBN: 978-1-4939-1660-3Published: 13 October 2014

  • Softcover ISBN: 978-1-4939-5525-1Published: 23 August 2016

  • eBook ISBN: 978-1-4939-1661-0Published: 11 October 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XI, 208

  • Number of Illustrations: 30 b/w illustrations, 40 illustrations in colour

  • Topics: Cancer Research, Apoptosis

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