Overview
- Includes ideas on how to approach a potential protein design project
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1216)
Access this book
Tax calculation will be finalised at checkout
Other ways to access
Table of contents (14 protocols)
Keywords
- binary-patterned protein libraries
- fluorinated proteins
- functional sites of proteins
- ketol-acid reductoisomerase
- metals
- monomeric β-hairpin
- non-fluorinated analogous structures
- protein variants
- structure-forming elements
- symmetric protein folds
- synthetic
- unnatural amino acids
- β-sheet peptides
- Protein Structure
About this book
Protein Design: Method and Applications, Second Edition expands upon the previous edition with current, detailed ideas on how to approach a potential protein design project. With new chapters on metals as structure-forming elements and functional sites, the design and characterization of fluorinated proteins, top-down symmetric deconstruction and the design of protein libraries and novel or repurposed enzymes. Written in the highly successful Methods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Thorough and intuitive, Protein Design: Method and Applications, Second Edition provides a number of practical protocols and instructive reviews to aid in the creation of new experiments.
Editors and Affiliations
Bibliographic Information
Book Title: Protein Design
Book Subtitle: Methods and Applications
Editors: Valentin Köhler
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-1486-9
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2014
Hardcover ISBN: 978-1-4939-1485-2Published: 11 September 2014
Softcover ISBN: 978-1-4939-4408-8Published: 17 September 2016
eBook ISBN: 978-1-4939-1486-9Published: 11 September 2014
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 2
Number of Pages: X, 279
Number of Illustrations: 21 b/w illustrations, 55 illustrations in colour
Topics: Protein Science, Computer Appl. in Life Sciences, Protein Structure