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  • Book
  • © 2014

In Situ Hybridization Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1211)

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Table of contents (21 protocols)

  1. Front Matter

    Pages i-xi
  2. MicroRNA In Situ Hybridization on Whole-Mount Preimplantation Embryos

    • Karen Goossens, Luc Peelman, Ann Van Soom
    Pages 15-25
  3. Whole-Mount In Situ Hybridization (WISH) Optimized for Gene Expression Analysis in Mouse Embryos and Embryoid Bodies

    • Eleni Dakou, Nele Vanbekbergen, Sara Corradi, Caroline R. Kemp, Erik Willems, Luc Leyns
    Pages 27-40
  4. Whole-Mount In Situ Hybridization Using DIG-Labeled Probes in Planarian

    • Agnieszka Rybak-Wolf, Jordi Solana
    Pages 41-51
  5. LNA-Based In Situ Hybridization Detection of mRNAs in Embryos

    • Diana K. Darnell, Parker B. Antin
    Pages 69-76
  6. Chromogen Detection of microRNA in Frozen Clinical Tissue Samples Using LNAâ„¢ Probe Technology

    • Boye Schnack Nielsen, Trine Møller, Kim Holmstrøm
    Pages 77-84
  7. MicroRNA In Situ Hybridization in Tissue Microarrays

    • Julia J. Turnock-Jones, John P. C. Le Quesne
    Pages 85-93
  8. Sensitive and Specific In Situ Hybridization for Early Drug Discovery

    • Pernille A. Usher, Elisabeth D. Galsgaard, Kimberly Kruse, Jishu Wang, Berit O. Krogh, Jette Mandelbaum et al.
    Pages 103-123
  9. Dual-Color Ultrasensitive Bright-Field RNA In Situ Hybridization with RNAscope

    • Hongwei Wang, Nan Su, Li-Chong Wang, Xingyong Wu, Son Bui, Allissa Nielsen et al.
    Pages 139-149
  10. Multiplexed miRNA Fluorescence In Situ Hybridization for Formalin-Fixed Paraffin-Embedded Tissues

    • Neil Renwick, Pavol Cekan, Claudia Bognanni, Thomas Tuschl
    Pages 171-187
  11. Quantitative Ultrasensitive Bright-Field RNA In Situ Hybridization with RNAscope

    • Hongwei Wang, Nan Su, Li-Chong Wang, Xingyong Wu, Son Bui, Allissa Nielsen et al.
    Pages 201-212

About this book

In Situ Hybridization Protocols, Fourth Edition contains 21 protocols that utilize the in situ hybridization technology to document or take advantage of the visualization of specific RNA molecules. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, In Situ Hybridization Protocols, Fourth Edition seeks to aid scientists in the further discovery of new RNA species and uncovering of their cellular functions.

Editors and Affiliations

  • Molecular Histology, Bioneer A/S, Horsholm, Denmark

    Boye Schnack Nielsen

Bibliographic Information

  • Book Title: In Situ Hybridization Protocols

  • Editors: Boye Schnack Nielsen

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-1459-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2014

  • Hardcover ISBN: 978-1-4939-1458-6Published: 13 September 2014

  • Softcover ISBN: 978-1-4939-5526-8Published: 22 September 2016

  • eBook ISBN: 978-1-4939-1459-3Published: 13 September 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 4

  • Number of Pages: XI, 275

  • Number of Illustrations: 4 b/w illustrations, 52 illustrations in colour

  • Topics: Human Genetics

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access