Overview
- Includes cutting-edge methods and protocols in the MiMB standard format
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 1188)
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Table of contents (24 protocols)
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Front Matter
Keywords
- Cell Culture
- Gram negative bacteria
- Gram positive bacteria
- RNAi-mediated gene knockdown
- SILAC labeling of the yeast Saccharomyces cerevisiae
- SILAC technology
- Trypanosoma brucei
- autotrophic organisms
- human protein complexes
- human splicosome
- metabolic labeling
- nuclear protein-protein interactions
- protein interactomes
- quantitative phosphoproteome analyses in C. elegans
- signaling networks
- time-resolved phosphoproteomics studies
About this book
Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC): Methods and Protocols provides a synopsis of a large array of different SILAC methods by presenting a set of protocols that have been established by renowned scientists and their working groups. These include methods and protocols for the labeling of various model organisms as well as advanced strategies relying on SILAC, e.g. for the analysis of protein interactions, the mapping of posttranslational modifications or the characterization of subcellular proteomes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC): Methods and Protocols will serve students and experienced scientists alike as a valuable reference of how to make use of the SILAC technology for their own research.
Editors and Affiliations
Bibliographic Information
Book Title: Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC)
Book Subtitle: Methods and Protocols
Editors: Bettina Warscheid
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-1142-4
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2014
Hardcover ISBN: 978-1-4939-1141-7Published: 25 July 2014
Softcover ISBN: 978-1-4939-5257-1Published: 20 September 2016
eBook ISBN: 978-1-4939-1142-4Published: 24 July 2014
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XIV, 372
Number of Illustrations: 38 b/w illustrations, 27 illustrations in colour
Topics: Protein Science, Proteomics