Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 1185)
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Table of contents (23 protocols)
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Front Matter
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Overview
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Front Matter
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Stem Cell Enrichment and Heterogeneity
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Front Matter
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Molecular and Cellular Characterization
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Front Matter
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In Vitro Assays and Differentiation
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Front Matter
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About this book
Updating and building upon previous editions, Hematopoietic Stem Cell Protocols, Third Edition provides up-to-date protocols from leading stem cell researchers. This in-depth volume presents a clear view of the landscape of assays available to the stem cell researcher working in the growing hematopoietic stem cell (HSC) field. A robust and active field, it is supported by an abundance of innovative mouse models and molecular tools for analysis of phenotypes and functions in mouse and human cells. Understanding more about hematopoietic stem cell biology is integral if these versatile cells are to be applied effectively to treat and cure a wide range of blood diseases. An introductory chapter puts the major contributions of the book into the proper perspective. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Essential for the laboratory-based researcher, Hematopoietic Stem Cell Protocols, Third Edition is a much needed technical resource in the critically important field of hematopoietic stem cell investigation.
Editors and Affiliations
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Department of Pediatrics, Aflac Cancer and Blood Disorders Center Emory University School of Medicine, Atlanta, USA
Kevin D. Bunting
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Department of Pediatrics, Emory University School of Medicine Aflac Cancer & Blood Disorders Ctr, Atlanta, USA
Cheng-Kui Qu
Bibliographic Information
Book Title: Hematopoietic Stem Cell Protocols
Editors: Kevin D. Bunting, Cheng-Kui Qu
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-1133-2
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2014
Hardcover ISBN: 978-1-4939-1132-5Published: 26 July 2014
Softcover ISBN: 978-1-4939-4757-7Published: 17 September 2016
eBook ISBN: 978-1-4939-1133-2Published: 25 July 2014
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 3
Number of Pages: XII, 364
Number of Illustrations: 10 b/w illustrations, 42 illustrations in colour
Topics: Cell Biology, Stem Cells