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  • © 2014

Eosinophils

Methods and Protocols

Editors:

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1178)

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Table of contents (26 protocols)

  1. Front Matter

    Pages i-xii
  2. Eosinophil Overview: Structure, Biological Properties, and Key Functions

    • Paige Lacy, Helene F. Rosenberg, Garry M. Walsh
    Pages 1-12
  3. Eosinophil Purification from Peripheral Blood

    • Praveen Akuthota, Kelsey Capron, Peter F. Weller
    Pages 13-20
  4. Eosinophil Purification from Human Bone Marrow

    • Tina W. Wong, Diane F. Jelinek
    Pages 21-27
  5. CD34+ Eosinophil-Lineage-Committed Cells in the Mouse Lung

    • Apostolos Bossios, Madeleine RÃ¥dinger
    Pages 29-43
  6. Eosinophil Cell Lines

    • Kenji Ishihara
    Pages 45-51
  7. Eosinophil Intracellular Signalling: Apoptosis

    • Pinja Ilmarinen, Eeva Moilanen, Hannu Kankaanranta
    Pages 71-80
  8. Identification of Human Eosinophils in Whole Blood by Flow Cytometry

    • Caroline Ethier, Paige Lacy, Francis Davoine
    Pages 81-92
  9. Isolation and Functional Assessment of Eosinophil Crystalloid Granules

    • Renata Baptista-dos-Reis, Valdirene S. Muniz, Josiane S. Neves
    Pages 93-100
  10. Eosinophil Chemotaxis

    • Gordon Dent
    Pages 101-110
  11. Eosinophil Shape Change and Secretion

    • Lian Willetts, Sergei I. Ochkur, Elizabeth A. Jacobsen, James J. Lee, Paige Lacy
    Pages 111-128
  12. Human Eosinophil Adhesion and Receptor Expression

    • Colleen S. Curran
    Pages 129-141
  13. Adhesion of Eosinophils to Endothelial Cells or Substrates Under Flow Conditions

    • Viktoria Konya, Miriam Peinhaupt, Akos Heinemann
    Pages 143-156
  14. Human Eosinophil Transmigration

    • Stanislawa Bazan-Socha, Joanna Zuk, Bogdan Jakiela, Jacek Musial
    Pages 157-164
  15. Measurement of Eosinophil Kinetics in Healthy Volunteers

    • Neda Farahi, Chrystalla Loutsios, Rosalind P. Simmonds, Linsey Porter, Daniel Gillett, Sarah Heard et al.
    Pages 165-176
  16. Assays of Eosinophil Apoptosis and Phagocytic Uptake

    • David A. Dorward, Sidharth Sharma, Ana L. Alessandri, Adriano G. Rossi, Christopher D. Lucas
    Pages 177-195
  17. Qualitative and Quantitative Studies of Eosinophils in Parasitic Infections

    • Masataka Korenaga, Fabrizio Bruschi
    Pages 203-213
  18. Interactions of Eosinophils with Nerves

    • Quinn R. Roth-Carter, David B. Jacoby, Zhenying Nie
    Pages 215-229

About this book

In Eosinophil: Methods and Protocols, experts in the field of eosinophil biology comprehensively provide detailed methodological insight into the study of this fascinating cell. This book is aimed at a diverse range of basic and clinical scientists who wish to work with eosinophils or who require an update of their knowledge or to gain the information required to study a function of the eosinophil different to their current area of enquiry.​ Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Eosinophil: Methods and Protocols seeks to aid scientist in the discovery of new hypotheses and for further examination of this intriguing cell.

Editors and Affiliations

  • Division of Applied Medicine, University of Aberdeen Institute of Medical Sciences, Aberdeen, United Kingdom

    Garry M. Walsh

Bibliographic Information

  • Book Title: Eosinophils

  • Book Subtitle: Methods and Protocols

  • Editors: Garry M. Walsh

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-1016-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2014

  • Hardcover ISBN: 978-1-4939-1015-1Published: 03 July 2014

  • Softcover ISBN: 978-1-4939-5509-1Published: 23 August 2016

  • eBook ISBN: 978-1-4939-1016-8Published: 01 July 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 324

  • Number of Illustrations: 25 b/w illustrations, 39 illustrations in colour

  • Topics: Immunology

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access