Overview
- Editors:
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E. J. Murray
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Roche Products Ltd., Welwyn Garden City, UK
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Table of contents (32 protocols)
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Front Matter
Pages i-xiii
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- Rémy Aubin, Michael Weinfeld, Malcolm C. Paterson
Pages 3-13
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- Hiroto Okayama, Claudia Chen
Pages 15-21
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- Richard A. Lake, Michael J. Owen
Pages 23-33
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- Remy Aubin, Michael Weinfeld, Malcolm C. Paterson
Pages 35-43
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- Janet M. Shipley, Denise Sheer
Pages 99-106
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- Frank L. Graham, Ludvik Prevec
Pages 109-128
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- Murk J. Bailey, Robert D. Possee
Pages 147-168
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- Marie-Louise Hammurskjöld
Pages 169-180
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- Jay P. Morgenstern, Hartmut Land
Pages 181-206
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- Grant R. MacGregor, Gamy P. Nolan, Steven Fiering, Mario Roederer, Leonard A. Herzenberg
Pages 217-235
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- Robert F. Santerre, Jenna D. Walls, Brian W. Grinnell
Pages 245-256
About this book
Biology is the study of living things. The classical approach might be described as holistic and descriptive, whereas the modern molecular - proach aims to be investigative, reductionist, and mechanistic . Genes contain all the information for the structure of all living things ; thus, the understanding of how genes are regulated is an important step toward understanding the nature of living things. The study of gene regulation has been made more tractable by the design of simple expe- mental models in which a single gene can be isolated from the milieu of the organism. The new science of molecular biology has introduced techniques that permit the design of such experimental models. In - sence, the genome of the organism is dissected in such a manner that specific genes may now be introduced into an appropriate cell line . Subsequent analysis of the proteins expressed from the genes under study results in the identification of the regulatory DNA sequences .
Reviews
Popular....Friendly guide...follows the established format...combining recipes and protocols with background information and useful troubleshooting hints....Gene Transfer and Expression Protocols is pitched at just the right level and is a worthy addition....A worthwhile and accurate manual written by the right people. -Society for General Microbiology Quarterly
Editors and Affiliations
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Roche Products Ltd., Welwyn Garden City, UK
E. J. Murray