Skip to main content
SpringerLink
Log in

Transforming Growth Factor-Beta Protocols

  • Book
  • © 2000

Overview

Editors:
  1. Philip H. Howe

    1. The Cleveland Clinic Foundation, Cleveland, OH

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Biology (MIMB, volume 142)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (13 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. In Vitro Assays for Measuring TGFβ Growth Stimulation and Inhibition

    • Maryanne Edens, Edward B. Leof
    Pages 1-11

  3. Measurements of Active TGFβ Generated by Culture Cells

    • Roberta Mazzieri, John S. Munger, Daniel B. Rifkin
    Pages 13-27

  4. Methods for Iodination of Active TGFβ, TGFβ Receptor Crosslinking and Immunoprecipitation of TGFβ-Receptor Complexes

    • David Danielpour
    Pages 29-37

  5. Detection of TGFβ in Body Fluids and Tissues

    • Andrew H. Limper
    Pages 39-54

  6. Analysis of TGFβ-Mediated Synthesis of Extracellular Matrix Components

    • Barbara A. Hocevar, Philip H. Howe
    Pages 55-65

  7. Characterization of SMAD Phosphorylation and SMAD/Receptor Interaction

    • Arun Mehra, Liliana Attisano, Jeffrey L. Wrana
    Pages 67-78

  8. Promoter Analysis of TGFβ-Responsive Genes by Transient Transfection and Deletional/Mutational Analysis

    • Ge Jin, Philip H. Howe
    Pages 79-95

  9. Regulation of AP-1 Activity by TGFβ

    • Barbara A. Hocevar, Philip H. Howe
    Pages 97-108

  10. CDK Pathway: Cyclin-Dependent Kinases (cdks) and Cyclin-Dependent Kinase Inhibitors

    • Diana M. Gitig, Andrew Koff
    Pages 109-123

  11. Activation of the Mitogen-Activated Protein Kinase Pathway by TGFβ

    • Jianbo Yue, Kathleen M. Mulder
    Pages 125-131

  12. 11 Detection of TGFβ Type II Receptor Hotspot Mutations: The BAT-RII Assay

    • Lois L. Myeroff, Sanford Markowitz
    Pages 133-137

  13. Mutation Detection in the TGFβ Receptors and SMAD Genes: RT-PCR and Sequencing

    • Lois L. Myeroff, Hongmei He, Stephen P. Fink, Sanford Markowitz
    Pages 139-147

  14. Analysis of TGFβ-Inducible Apoptosis

    • Thomas L. Brown, Supriya Patil, Philip H. Howe
    Pages 149-167

  15. Back Matter

    Pages 169-175
    Download chapter PDF
Back to top

About this book

Philip H. Howe and a group of well-versed experimentalists present the first major volume a collection of indispensable classic and cutting-edge TGFß assays. Described in great detail to ensure robust and successful results, these readily reproducible techniques range from the growth inhibition assay for TGFß to methods for monitoring its interactions with the mediating proteins. Extensive notes discuss potential pitfalls and provide tips on how to avoid failures, and throughout, emphasis is given to detailing those technical steps critical for experimental success that are often omitted in the primary literature.
Concise and highly practical, Transforming Growth Factor-Beta Protocols provides today's molecular and cell biologists-both expert and novice-with time-tested methods for the identification and analysis of the signal transduction pathways by which TGFß induces and modulates physiological behavior.

Reviews

"This book would be most useful to a lab researcher who had not previously been studying TGF-ß. Having all the practical protocols for preparing, assaying, and studying this important molecule in a small volume could be very useful for such a researcher. "-Doody's Health Science Book Review Journal

Editors and Affiliations

  • The Cleveland Clinic Foundation, Cleveland, OH

    Philip H. Howe

Bibliographic Information

  • Book Title: Transforming Growth Factor-Beta Protocols

  • Editors: Philip H. Howe

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592590535

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2000

  • Hardcover ISBN: 978-0-89603-646-8Published: 17 March 2000

  • Softcover ISBN: 978-1-4899-4398-9Published: 08 August 2013

  • eBook ISBN: 978-1-59259-053-7Published: 05 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 165

  • Topics: Cell Biology

Publish with us

Policies and ethics

Back to top

Search

Navigation